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HILIC mode separation of polar compounds by monolithic silica capillary columns coated with polyacrylamide

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Abstract

HILIC mode columns were prepared by an on-column polymerization of acrylamide on a monolithic silica capillary column modified with N-(3-trimethoxysilylpropyl)methacrylamide as the anchor group. The products showed HILIC mode retention characteristics with three times greater permeability and slightly higher column efficiency compared to a commercially available amide-type HILIC column packed with 5-μm particles. The selectivity of the monolithic silica-based column was similar to that of the particulate column for each group of solutes towards nucleosides, nucleic bases and carbohydrate derivatives, although a considerable difference was observed in the selectivity for the solute groups. Although the retention of solutes based on the polar functionality was much smaller with the monolithic silica columns, which had a smaller phase ratio, than with the particle-packed column, the former can achieve better separation utilizing the high permeability and higher column efficiencies of a longer column.

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Acknowledgements

This work was supported by two Grants-in-Aid for Scientific Research funded by the Ministry of Education, Sports, Culture, Science and Technology, No. 14340234 and No. 14740403.

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Authors and Affiliations

  1. Department of Polymer Science and Engineering, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto, 606-8585, Japan

    Tohru Ikegami, Hiroshi Fujita, Kanta Horie, Ken Hosoya & Nobuo Tanaka

Authors
  1. Tohru Ikegami
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  2. Hiroshi Fujita
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  3. Kanta Horie
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  4. Ken Hosoya
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  5. Nobuo Tanaka
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Correspondence to Tohru Ikegami.

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Ikegami, T., Fujita, H., Horie, K. et al. HILIC mode separation of polar compounds by monolithic silica capillary columns coated with polyacrylamide. Anal Bioanal Chem 386, 578–585 (2006). https://doi.org/10.1007/s00216-006-0606-z

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  • DOI: https://doi.org/10.1007/s00216-006-0606-z

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