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Measurement of antibody binding to protein immobilized on gold nanoparticles by localized surface plasmon spectroscopy

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Abstract

Antibody binding to bovine serum albumin (BSA) and human serum albumin (HSA) immobilized onto gold nanoparticles was studied by means of localized surface plasmon resonance (LSPR) spectroscopy. Amine-modified glass was prepared by self-assembly of amine-terminated silane on substrate, and gold (Au) nanoparticles were deposited on the amine-modified glass substrate. Au nanoparticles deposited on the glass surface were functionalized by BSA and HSA. BSA immobilization was confirmed by LSPR spectroscopy in conjunction with surface-enhanced Raman scattering spectroscopy. Then, LSPR response attributable to the binding of anti-BSA and anti-HSA to BSA- and HSA-functionalized Au nanoparticles, respectively, was examined. Anti-HSA at levels larger than ∼10 nM could be detected by HSA-immobilized chips with LSPR optical response, which was saturated at concentrations greater than ∼650 nM of anti-HSA.

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References

  1. Bohren CF, Huffman DR (1983) Absorption and scattering of light by small metal particles. Wiley, New York

    Google Scholar 

  2. Kalkbrenner T, Håkanson U, Sandoghdar V (2004) Nano Lett 4:2309–2314

    Article  CAS  Google Scholar 

  3. Elghanian R, Storhoff JJ, Mucic RC, Letsinger RL, Mirkin CA (1997) Science 277:1078–1081

    Article  CAS  Google Scholar 

  4. Rich AL, Myszka DG (2000) Curr Opin Biotechnol 11:54–61

    Article  CAS  Google Scholar 

  5. Brockman JM, Nelson BP, Corn RM (2000) Annu Rev Phys Chem 51:41–53

    Article  CAS  Google Scholar 

  6. Nath N, Chilkoti A (2002) Anal Chem 74:504–509

    Article  CAS  Google Scholar 

  7. Nath N, Chilkoti A (2004) Anal Chem 76:5370–5378

    Article  CAS  Google Scholar 

  8. Mitsui K, Handa Y, Kajikawa K (2004) Appl Phys Lett 85:4231–4233

    Article  CAS  Google Scholar 

  9. Cheng S-F, Chau L-K (2003) Anal Chem 75:16–21

    Article  CAS  Google Scholar 

  10. Green NM, Toms EJ (1973) Biochem J 133:687–700

    CAS  Google Scholar 

  11. Kambhampati D (ed) (2004) Protein microarray technology. Wiley-VCH Verlag, Weinheim

  12. Peters T (1995) All about albumin: biochemistry, genetics, and medical applications. Academic Press, San Diego

    Google Scholar 

  13. Carter DC, Ho JX (1994) Adv Protein Chem 45:152–203

    Google Scholar 

  14. Graber KC, Freeman G, Hommer MB, Natan MJ (1995) Anal Chem 67:735–743

    Article  Google Scholar 

  15. Nie S, Emory SR (1997) Science 275:1102–1106

    Article  CAS  Google Scholar 

  16. Félidj N, Aubard J, Lévi G, Krenn JR, Hohenau A, Schider G, Leiner A, Aussenegg FR (2004) Appl Phys Lett 82:3095–3097

    Article  Google Scholar 

  17. Bonifacio A, Sneppen L, Gooijer C, Zwan G (2004) Langmuir 20:5858–5864

    Article  CAS  Google Scholar 

  18. Cavalu S, Cinta Pinzaru S, Leopold N, Kieefer W (2001) Biopolymers 62:341–348

    Article  CAS  Google Scholar 

  19. Nisonoff A (ed) (1982) Introduction to molecular immunology. Sinauer, Sunderland

  20. Ramachandran N, Hainsworth E, Bhullar B, Eisenstein S, Rosen B, Lau AY, Walter JC, LaBaer J (2004) Science 305:86–90

    Article  CAS  Google Scholar 

  21. Voet D, Voet JG (1995) Biochemistry, 2nd edn. Wiley, New York

    Google Scholar 

Download references

Acknowledgements

This work was supported by grants to K.F. from Foundation Advanced Technology Institute, Izumi Science and Technology (H16-J-56), and “Research for Promoting Technological Seeds” of Japan Science and Technology Agency.

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Authors and Affiliations

  1. Department of Materials Process & Applied Chemistry for Environments, Faculty of Engineering and Resource Science, Akita University, Tegata Gakuen-cho, Akita, 010-8502, Japan

    Kazuhiko Fujiwara, Hideaki Itoh, Erika Nakahama & Nobuaki Ogawa

  2. Department of Chemistry, Graduate School of Science, Osaka University, Toyonaka, Osaka, 560-0043, Japan

    Hitoshi Watarai

Authors
  1. Kazuhiko Fujiwara
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  2. Hitoshi Watarai
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  3. Hideaki Itoh
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  4. Erika Nakahama
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  5. Nobuaki Ogawa
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Corresponding author

Correspondence to Kazuhiko Fujiwara.

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Fujiwara, K., Watarai, H., Itoh, H. et al. Measurement of antibody binding to protein immobilized on gold nanoparticles by localized surface plasmon spectroscopy. Anal Bioanal Chem 386, 639–644 (2006). https://doi.org/10.1007/s00216-006-0559-2

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  • DOI: https://doi.org/10.1007/s00216-006-0559-2

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