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Miniaturized platforms for the detection of single-nucleotide polymorphisms

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Abstract

Conventional methods for detecting single-nucleotide polymorphisms (SNPs), the most common form of genetic variation in human beings, are mostly limited by their analysis time and throughputs. In contrast, advances in microfabrication technology have led to the development of miniaturized platforms that can potentially provide rapid high-throughput analysis at small sample volumes. This review highlights some of the recent developments in the miniaturization of SNP detection platforms, including microarray-based, bead-based microfluidic and microelectrophoresis-based platforms. Particular attention is paid to their ease of fabrication, analysis time, and level of throughput.

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Authors and Affiliations

  1. Division of Environmental Science and Engineering, National University of Singapore, 21 Lower Kent Ridge Road, 119077, Singapore, Singapore

    Johnson Kian-Kok Ng & Wen-Tso Liu

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  1. Johnson Kian-Kok Ng
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  2. Wen-Tso Liu
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Correspondence to Wen-Tso Liu.

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Ng, J.KK., Liu, WT. Miniaturized platforms for the detection of single-nucleotide polymorphisms. Anal Bioanal Chem 386, 427–434 (2006). https://doi.org/10.1007/s00216-006-0552-9

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  • DOI: https://doi.org/10.1007/s00216-006-0552-9

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