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31P NMR study on the autophosphorylation of insulin receptors in the plasma membrane

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Abstract

A nonradioactive 31P nuclear magnetic resonance (NMR) spectroscopy protocol has been developed and used to investigate in vitro autophosphorylation of insulin receptors. Optimum experimental conditions have been explored, and the effects of Mn2+ and phosphocreatine (PCr) on the determination of the phosphorylation reaction have been assayed. The method was used to monitor the time courses of the phosphorylation reaction in solution. The results from this NMR study were in agreement with observations of insulin receptor phosphorylation made by using Western blotting.

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Acknowledgements

This work was supported by grants from the National Natural Science Foundation of China (Project No. 20371018).

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Authors and Affiliations

  1. Department of Chemistry, Huazhong University of Science and Technology, Wuhan, 430074, People’s Republic of China

    Yili Ge, Hong Peng & Kaixun Huang

  2. Faculty of Chemistry and Chemical Engineering, Hubei University, Wuhan, 430062, People’s Republic of China

    Yili Ge

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  1. Yili Ge
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  2. Hong Peng
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  3. Kaixun Huang
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Correspondence to Kaixun Huang.

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Ge, Y., Peng, H. & Huang, K. 31P NMR study on the autophosphorylation of insulin receptors in the plasma membrane. Anal Bioanal Chem 385, 834–839 (2006). https://doi.org/10.1007/s00216-006-0507-1

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  • DOI: https://doi.org/10.1007/s00216-006-0507-1

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