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Application of anodized aluminum in fluorescence detection of biological species

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Abstract

Thin nanoporous alumina obtained by anodization of aluminum films offers promising advantages for application in fluorescence-based biological sensors including convenient preparation, increased density of binding sites, and improved collection efficiency of fluorescence. These advantages are illustrated in the detection of streptavidin using biotin covalently bound to the surface of alumina nanopores. Fluorescence intensity enhancement as high as 7 times is observed in nanopores in comparison to flat glass surface.

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Acknowledgements

We are grateful to NIH for financial support (NIH SCORE GM08136) and would like to thank Dr. Dmitri Brevnov (University of New Mexico) for help with SEM.

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Authors and Affiliations

  1. Department of Chemistry and Biochemistry, New Mexico State University, Las Cruces, NM, 88003, USA

    Pavel Takmakov, Ivan Vlassiouk & Sergei Smirnov

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  1. Pavel Takmakov
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  2. Ivan Vlassiouk
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  3. Sergei Smirnov
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Correspondence to Sergei Smirnov.

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Takmakov, P., Vlassiouk, I. & Smirnov, S. Application of anodized aluminum in fluorescence detection of biological species. Anal Bioanal Chem 385, 954–958 (2006). https://doi.org/10.1007/s00216-006-0504-4

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  • DOI: https://doi.org/10.1007/s00216-006-0504-4

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