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Longitudinal diffusion behavior of hemicyanine dyes across phospholipid vesicle membranes as studied by second-harmonic generation and fluorescence spectroscopies

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Abstract

The adsorption and longitudinal diffusion behaviors of a series of hemicyanine dyes to phospholipid vesicle membranes were studied by second-harmonic generation (SHG) and fluorescence spectroscopies. It was observed that the longitudinal diffusion of cationic hemicyanine dyes takes place immediately after the initial adsorption of these dyes to the outer surface of the vesicle membrane. In contrast, hardly any amount of a zwitterionic hemicyanine dye with a sulfonate group diffused across the vesicle membrane within the measurement time (<2000 s). Based on the difference in the time-course responses of SHG and fluorescence spectroscopies for all of the hemicyanine dyes tested, we propose that hydration of the sulfonate group is mainly responsible for the low diffusivity of the zwitterionic hemicyanine dye.

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Acknowledgements

We thank Dr. Tsunenobu Onodera and Dr. Hitoshi Kasai for measuring the dynamic light scattering. This work was supported in part by a Grant-in-Aid for Scientific Research (A), No. 17205009 from the Ministry of Education, Culture, Sports, Science and Technology, Japan, and the Kao Foundation for Arts and Sciences.

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Authors and Affiliations

  1. Department of Chemistry, Graduate School of Science, Tohoku University, Aoba-ku, Sendai, 980-8578, Japan

    Akira Yamaguchi, Masaki Nakano, Kimihisa Nochi, Tomohisa Yamashita, Kotaro Morita & Norio Teramae

  2. PRESTO, Japan Science and Technology Agency, Kawaguchi, 332-0012, Japan

    Akira Yamaguchi

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  1. Akira Yamaguchi
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  2. Masaki Nakano
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  3. Kimihisa Nochi
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  4. Tomohisa Yamashita
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  5. Kotaro Morita
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  6. Norio Teramae
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Correspondence to Akira Yamaguchi.

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Yamaguchi, A., Nakano, M., Nochi, K. et al. Longitudinal diffusion behavior of hemicyanine dyes across phospholipid vesicle membranes as studied by second-harmonic generation and fluorescence spectroscopies. Anal Bioanal Chem 386, 627–632 (2006). https://doi.org/10.1007/s00216-006-0470-x

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  • DOI: https://doi.org/10.1007/s00216-006-0470-x

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