On-column sample enrichment for the high-sensitivity sheath-flow CE-MS analysis of peptides
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A sample enrichment technique to increase sensitivity in capillary electrophoresis–mass spectrometry (CE-MS) is described. Peptides or glycopeptides are retained and concentrated on a short (3–5-mm) reversed-phase (C18) packed-bed situated in the fused-silica separation capillary and are subsequently released for electrophoretic separation by injection of an organic elutant. The concentration limits of detection are in the high picomolar range with a sheath-flow CE-MS interface.
KeywordsCapillary electrophoresis Mass spectrometry Peptides In-line preconcentration
This work was supported by a research grant to K.S. from the Institute for the Promotion of Innovation by Science and Technology in Flanders (IWT-Vlaanderen) and by the Fund for Scientific Research-Flanders (FWO-Vlaanderen) through project G.0312.02 to J.V.B. and B.D. The authors gratefully acknowledge Applera Benelux for instrumental support.
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