Analytical and Bioanalytical Chemistry

, Volume 385, Issue 4, pp 671–677 | Cite as

On-column sample enrichment for the high-sensitivity sheath-flow CE-MS analysis of peptides

  • K. Sandra
  • F. Lynen
  • B. Devreese
  • J. Van BeeumenEmail author
  • P. Sandra
Short Communication


A sample enrichment technique to increase sensitivity in capillary electrophoresis–mass spectrometry (CE-MS) is described. Peptides or glycopeptides are retained and concentrated on a short (3–5-mm) reversed-phase (C18) packed-bed situated in the fused-silica separation capillary and are subsequently released for electrophoretic separation by injection of an organic elutant. The concentration limits of detection are in the high picomolar range with a sheath-flow CE-MS interface.


Capillary electrophoresis Mass spectrometry Peptides In-line preconcentration 



This work was supported by a research grant to K.S. from the Institute for the Promotion of Innovation by Science and Technology in Flanders (IWT-Vlaanderen) and by the Fund for Scientific Research-Flanders (FWO-Vlaanderen) through project G.0312.02 to J.V.B. and B.D. The authors gratefully acknowledge Applera Benelux for instrumental support.


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Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • K. Sandra
    • 1
    • 3
  • F. Lynen
    • 2
  • B. Devreese
    • 1
  • J. Van Beeumen
    • 1
    Email author
  • P. Sandra
    • 2
  1. 1.Laboratory of Protein Biochemistry and Protein EngineeringGhent UniversityGentBelgium
  2. 2.Pfizer Analytical Research Centre (PARC)Ghent UniversityGhentBelgium
  3. 3.Peakadilly nv.Zwijnaarde/GhentBelgium

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