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Analytical and Bioanalytical Chemistry

, Volume 385, Issue 4, pp 671–677 | Cite as

On-column sample enrichment for the high-sensitivity sheath-flow CE-MS analysis of peptides

  • K. Sandra
  • F. Lynen
  • B. Devreese
  • J. Van BeeumenEmail author
  • P. Sandra
Short Communication

Abstract

A sample enrichment technique to increase sensitivity in capillary electrophoresis–mass spectrometry (CE-MS) is described. Peptides or glycopeptides are retained and concentrated on a short (3–5-mm) reversed-phase (C18) packed-bed situated in the fused-silica separation capillary and are subsequently released for electrophoretic separation by injection of an organic elutant. The concentration limits of detection are in the high picomolar range with a sheath-flow CE-MS interface.

Keywords

Capillary electrophoresis Mass spectrometry Peptides In-line preconcentration 

Notes

Acknowledgements

This work was supported by a research grant to K.S. from the Institute for the Promotion of Innovation by Science and Technology in Flanders (IWT-Vlaanderen) and by the Fund for Scientific Research-Flanders (FWO-Vlaanderen) through project G.0312.02 to J.V.B. and B.D. The authors gratefully acknowledge Applera Benelux for instrumental support.

References

  1. 1.
    Hernandez-Borges J, Neusüβ C, Cifuentes A, Pelzing M (2004) Electrophoresis 25:2257–2281CrossRefGoogle Scholar
  2. 2.
    Kaiser T, Hermann A, Kielstein JT, Wittke S, Bartel S, Krebs R, Hausadel F, Hillman M, Golovko I, Koester P, Haller H, Weissinger EM, Flisser D, Mischak H (2003) J Chromatogr A 1013:157–171CrossRefGoogle Scholar
  3. 3.
    Moini M (2002) Anal Bioanal Chem 373:466–480CrossRefGoogle Scholar
  4. 4.
    Monton MRN, Terabe S (2005) Anal Sci 21:5–13CrossRefGoogle Scholar
  5. 5.
    Sassi AP, Andel F 3rd, Bitter HM, Brown MP, Chapman RG, Espiritu J, Greenquist AC, Guyon I, Horchi-Alegre M, Stults KL, Wainright A, Heller JC, Stults JT (2005) Electrophoresis 26:1500–1512CrossRefGoogle Scholar
  6. 6.
    Simpson DC, Smith RD (2005) Electrophoresis 26:1291–1305CrossRefGoogle Scholar
  7. 7.
    Wittke S, Fliser D, Haubitz M, Bartel S, Krebs R, Hausadel F, Hillmann M, Golovko I, Koester P, Haller H, Kaiser T, Mischak H, Wissinger EM (2003) J Chromatogr A 1013:173–181CrossRefGoogle Scholar
  8. 8.
    Stroink T, Paarlberg E, Waterval JC, Bult A, Underberg WJ (2001) Electrophoresis 22:2375–2383CrossRefGoogle Scholar
  9. 9.
    Veraart JR, Lingeman H, Brinkman UA (1999) J Chromatogr A 856:483–514CrossRefGoogle Scholar
  10. 10.
    Wu X (2003) Trends Anal Chem 22:48–58CrossRefGoogle Scholar
  11. 11.
    Monton MN, Terabe S (2004) J Chromatogr A 1032:203–211CrossRefGoogle Scholar
  12. 12.
    Neusüβ C, Pelzing M, Macht M (2002) Electrophoresis 23:3149–3159CrossRefGoogle Scholar
  13. 13.
    Guzman NA, Trebilcock MA, Advis JP (1991) J Liq Chromatogr 14:997–1015CrossRefGoogle Scholar
  14. 14.
    Strausbauch MA, Landers JP, Wettstein PJ (1996) Anal Chem 68:306–314CrossRefGoogle Scholar
  15. 15.
    Barroso MB, de Jong AP (1999) J Am Soc Mass Spectrom 10:1271–1278CrossRefGoogle Scholar
  16. 16.
    Bateman KP, White RL, Thibault P (1998) J Mass Spectrom 33:1109–1123CrossRefGoogle Scholar
  17. 17.
    Figeys D, Ducret A, Yates JR III, Aebersold R (1996) Nat Biotechnol 14:1579–1583CrossRefGoogle Scholar
  18. 18.
    Figeys D, Ducret A, Aebersold R (1997) J Chromatogr A 763:295–306CrossRefGoogle Scholar
  19. 19.
    Herring CJ, Qin J (1999) Rapid Commun Mass Spectrom 13:1–7CrossRefGoogle Scholar
  20. 20.
    Janini GM, Zhou M, Yu LR, Blonder J, Gignac M, Conrads TP, Issaq HJ, Veenstra TD (2003) Anal Chem 75:5984–5993CrossRefGoogle Scholar
  21. 21.
    Pelzing M, Neusüβ C (2005) Electrophoresis 26:2717–2728CrossRefGoogle Scholar
  22. 22.
    Tomlinson AJ, Guzman NA, Naylor S (1995) J Capillary Electrophor 2:247–266Google Scholar
  23. 23.
    Tomlinson AJ, Naylor S (1995) J Capillary Electrophor 2:225–233Google Scholar
  24. 24.
    Tomlinson AJ, Braddock WD, Benson LM, Oda RP, Naylor S (1995) J Chromatogr B 669:67–73CrossRefGoogle Scholar
  25. 25.
    Waterval JCM, Hommels G, Teeuwsen J, Bult A, Lingeman H, Underberg WJM (2000) Electrophoresis 21:2851–2858CrossRefGoogle Scholar
  26. 26.
    Waterval JCM, Bertebreurtje P, Lingeman H, Versluis C, Heck AJR, Bult A, Underberg WJM (2001) Electrophoresis 22:2701–2708CrossRefGoogle Scholar
  27. 27.
    Waterval JCM, Hommels G, Bertebreurtje P, Versluis C, Heck AJR, Bult A, Lingeman H, Underberg WJM (2001) Electrophoresis 22:2709–2716CrossRefGoogle Scholar
  28. 28.
    Lynen F, Buica A, de Villiers A, Crouch A, Sandra P (2005) J Sep Sci 28:1539–1549CrossRefGoogle Scholar
  29. 29.
    De Vriendt K, Theunissen S, Carpentier W, De Smet L, Devreese B, Van Beeumen J (2005) Proteomics 5:1308–1316CrossRefGoogle Scholar
  30. 30.
    Zimina TM, Smith RM, Myers P (1997) J Chromatogr A 758:191–197CrossRefGoogle Scholar
  31. 31.
    Sandra K, Devreese B, Stals I, Claeyssens M, Van Beeumen J (2004) J Am Soc Mass Spectrom 15:413–423CrossRefGoogle Scholar
  32. 32.
    Kelly JF, Ramaley L, Thibault P (1997) Anal Chem 69:51–60CrossRefGoogle Scholar
  33. 33.
    Sandra K, Stals I, Sandra P, Claeyssens M, Van Beeumen J, Devreese B (2004) J Chromatogr A 1058:263–272Google Scholar
  34. 34.
    Ross GA (2001) LC-GC 14:45–49Google Scholar

Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • K. Sandra
    • 1
    • 3
  • F. Lynen
    • 2
  • B. Devreese
    • 1
  • J. Van Beeumen
    • 1
    Email author
  • P. Sandra
    • 2
  1. 1.Laboratory of Protein Biochemistry and Protein EngineeringGhent UniversityGentBelgium
  2. 2.Pfizer Analytical Research Centre (PARC)Ghent UniversityGhentBelgium
  3. 3.Peakadilly nv.Zwijnaarde/GhentBelgium

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