Analytical and Bioanalytical Chemistry

, Volume 385, Issue 4, pp 686–691 | Cite as

Short polystyrene monolith-fritted micro-liquid chromatography columns for rapid isocratic analysis of pharmaceuticals direct from plasma

  • Cristina Legido-QuigleyEmail author
  • Norman W. Smith
Original Paper


The manufacture of micro-HPLC columns with combined stationary phases, a body of 3.5-μm XTerra-C18 particles, and poly(styrene–divinylbenzene) (PS–DVB) frits is described in detail. The efficiency of the columns was assessed by rapid separation of neutral and acid compound mixtures. Direct analysis of some pharmaceuticals in plasma resulted in lower limits of detection (LOD) for salmeterol xinofate of 12.5 nanograms on-column.


Monolith frit Stationary phases MicroLC 



This work was performed thanks to a CASE award grant provided by GlaxoSmithKline (GSK) in the UK. We thank Bob Biddlecombe for kindly providing the plasma samples.


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Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  1. 1.Microseparations Group, Department of PharmacyKing’s College LondonLondonUK
  2. 2.Centre for Analytical Sciences, Department of ChemistryImperial College LondonLondonUK

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