Advertisement

Analytical and Bioanalytical Chemistry

, Volume 385, Issue 4, pp 686–691 | Cite as

Short polystyrene monolith-fritted micro-liquid chromatography columns for rapid isocratic analysis of pharmaceuticals direct from plasma

  • Cristina Legido-QuigleyEmail author
  • Norman W. Smith
Original Paper

Abstract

The manufacture of micro-HPLC columns with combined stationary phases, a body of 3.5-μm XTerra-C18 particles, and poly(styrene–divinylbenzene) (PS–DVB) frits is described in detail. The efficiency of the columns was assessed by rapid separation of neutral and acid compound mixtures. Direct analysis of some pharmaceuticals in plasma resulted in lower limits of detection (LOD) for salmeterol xinofate of 12.5 nanograms on-column.

Keywords

Monolith frit Stationary phases MicroLC 

Notes

Acknowledgements

This work was performed thanks to a CASE award grant provided by GlaxoSmithKline (GSK) in the UK. We thank Bob Biddlecombe for kindly providing the plasma samples.

References

  1. 1.
    Worsford P, Townshend A, Poole C (2004) (eds) Encyclopaedia of Analytical Science, 2nd edn. Elsevier, Amsterdam, The NetherlandsGoogle Scholar
  2. 2.
    Timbrell JA (1998) Toxicology 129(1):1–12CrossRefGoogle Scholar
  3. 3.
    Chilton S, Godward RE, Carey PF (1995) J Pharm Bio Anal 13:165–169CrossRefGoogle Scholar
  4. 4.
    Boulet L (1994) Drugs 47:204–222CrossRefGoogle Scholar
  5. 5.
    Bush RK, Georgitis JW (1997) (eds) Handbook of Asthma and Rhinitis, 1st edn. Blackwell Science Ltd, Abingdon, UKGoogle Scholar
  6. 6.
    Currie GP, Stenback S, Lipworth BJ (2003) J Clin Pharmacol 56:11–17CrossRefGoogle Scholar
  7. 7.
    Currie GP, Syme-Grant NJ, McFarlane LC, Carey FA, Lipworth BJ (2003) Allergy 58:602–607CrossRefGoogle Scholar
  8. 8.
    Legido-Quigley C, Marlin ND, Melin V, Manz A, Smith NW (2003) Electrophoresis 24:917–944CrossRefGoogle Scholar
  9. 9.
    Legido-Quigley C, Smith NW (2004) J Chromatogr A 1042:61–68CrossRefGoogle Scholar
  10. 10.
    Coufal P, Cihak M, Suchankova J, Tesarova E, Bosakova Z, Stulik K (2002) J Chromatogr A 946:99–106CrossRefGoogle Scholar
  11. 11.
    Legido-Quigley C, Smith NW, Mallet D (2002) J Chromatogr A 976:11–18CrossRefGoogle Scholar
  12. 12.
    Spikmans V, Lane SJ, Smith NW (2000) Chromatographia 51:18CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  1. 1.Microseparations Group, Department of PharmacyKing’s College LondonLondonUK
  2. 2.Centre for Analytical Sciences, Department of ChemistryImperial College LondonLondonUK

Personalised recommendations