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Powerful preconcentration method for capillary electrophoresis and its application to analysis of ultratrace amounts of polycyclic aromatic hydrocarbons

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Abstract

For environmental analyses, a high-performance and powerful preconcentration system exceeding 1×107-fold was developed that was composed of a blue cotton method (solid extraction method)/homogeneous liquid–liquid extraction method/on-line concentration method for capillary electrophoresis (CE). This system was named the “triplex concentration system” and it was achieved by finding a new phase-separation phenomenon (homogeneous liquid–liquid extraction) from a water-miscible organic solvent. Parts per trillion levels of polycyclic aromatic hydrocarbons (PAHs) were used as model analytical targets in this study. With the proposed method, 20-L levels of environmental water could be preconcentrated up to 1×107-fold within a maximum of 1 h. The parts per trillion levels of PAHs were easily determined even using UV/CE, which has a serious sensitivity problem, and the detection limit of benzo[a]pyrene was 3.60 ppt. This system was also used as a practical monitoring method for the Miyata River (in Japan).

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Authors and Affiliations

  1. Laboratory of Physical and Chemical Science, Fukushima University, 1 Kanayagawa, Fukushima, 960-1296, Japan

    Yoshitaka Takagai

  2. Department of Biomolecular Functional Engineering, Faculty of Engineering, Ibaraki University, 4-12-1 Nakanarusawa, Hitachi, Ibaraki, 316-8511, Japan

    Ryoutaro Akiyama & Shukuro Igarashi

Authors
  1. Yoshitaka Takagai
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  2. Ryoutaro Akiyama
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  3. Shukuro Igarashi
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Correspondence to Shukuro Igarashi.

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Takagai, Y., Akiyama, R. & Igarashi, S. Powerful preconcentration method for capillary electrophoresis and its application to analysis of ultratrace amounts of polycyclic aromatic hydrocarbons. Anal Bioanal Chem 385, 888–894 (2006). https://doi.org/10.1007/s00216-006-0447-9

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  • DOI: https://doi.org/10.1007/s00216-006-0447-9

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