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Analytical and Bioanalytical Chemistry

, Volume 385, Issue 4, pp 737–741 | Cite as

A sequential injection fluorometric procedure for the determination of procaine in human blood and pharmaceuticals

  • Xu-Wei Chen
  • Xin Song
  • Jian-Hua WangEmail author
Original Paper

Abstract

An automated procedure for the assay of procaine hydrochloride in human blood and pharmaceuticals was developed using a sequential injection (SI) technique with fluorometric detection and fluorescamine as the fluorescence probe. A few microliters of fluorescamine and procaine hydrochloride solutions were used in the SI system leading to the formation of a derivative, which was then excited by a 400-nm LED and whose emitted fluorescence was monitored at a wavelength of 494 nm. A linear calibration graph was obtained with 10–200 ng mL−1 (procaine) by loading 10.0 μL of sample solution and 5.0 μL of fluorescamine solution (both 0.125 % m/v). A detection limit of 2.6 ng mL−1, defined as 3 times the blank standard deviation (3σ), was achieved along with a sampling frequency of 25 h−1 and a precision of 2.1 % RSD at the 50.0 ng mL−1 level. Procaine contents in injection solutions from various pharmaceutical manufactures were analyzed and reasonable agreement was achieved between the values obtained by using the present procedure and the documented spectrophotometry, and both were coincident with the nominal concentrations. In addition, the degradation of procaine in human blood was investigated. A fast degradation of procaine in human blood was observed for the first 30 min, while afterwards the degradation was retarded.

Keywords

Sequential injection Fluorometry Procaine degradation Fluorecamine 

Notes

Acknowledgements

The authors are indebted to the financial support from the National Natural Science Foundation of China (NSFC-20375007, 20575010, NSFC-RFBR joint program), the key project for scientific research from the Ministry of Education (105056), the Natural Science Foundation of Liaoning Province, China (20042011, 20051013), and the SRFDP program (20050145026).

References

  1. 1.
    The United States Pharmacopoeia Convention Inc., Rockville, MD, 23rd revision, vol II, 1995 p 1298Google Scholar
  2. 2.
    The British Pharmacopoeia, British Pharmacopoeia Commission, London, HMSO, vol I, 1993, p 544Google Scholar
  3. 3.
    Pharmacopoeia Commission, Ministry of Health, The Chinese Pharmacopoeia, vol 2, 5th edn, PRC Chemical Industry Press, Beijing, 1995, p 711Google Scholar
  4. 4.
    Murillo Pulgarin JA, Alanon Molina A, Rodriguez Flores J (1992) Anal Lett 25(7):1275–1288Google Scholar
  5. 5.
    Xu LX, Shen YX, Wang HY, Jiang JG, Xiao Y (2003) Spectrochim Acta Part A 59:3103–3110CrossRefGoogle Scholar
  6. 6.
    Liu LD, Liu Y, Wang HY, Sun Y, Ma L, Tang B (2000) Talanta 52:991–999CrossRefGoogle Scholar
  7. 7.
    Zhang XR, Baeyens WRG, Van der Weken G, Calokerinos AC, Imai K (1995) Anal Chim Acta 303:137–142CrossRefGoogle Scholar
  8. 8.
    Stevenson AJ, Weber MP, Todi F, Mendonca M, Fenwick JD, Young L, Kwong E, Chen F, Beaumier P, Timmings S, Woodard D, Kacew S (1992) J Anal Toxicol 16(2):93–96PubMedGoogle Scholar
  9. 9.
    Segura Carretero A, Cruces-Blanco C, Fernández Peinado S, El Bergmi R, Fernández Gutiérrez A (1999) J Pharm Biomed Anal 21:969–974CrossRefPubMedGoogle Scholar
  10. 10.
    Sanchez FC, Ramos Rubio AL, Cruces Blanco C, Hernandez Lopez M, Marquez Gomez JC, Carnero C (1988) Anal Chim Acta 205:139–147CrossRefGoogle Scholar
  11. 11.
    Mo ZH, Luo J, Long XH, Xia ZL (1997) Fresenius J Anal Chem 358:556–558CrossRefGoogle Scholar
  12. 12.
    Li NB, Chi YW, Wang J, Duan JP, Chen GN (2003) Luminescence 18:125–130CrossRefPubMedGoogle Scholar
  13. 13.
    Wang JH, Hansen EH (2002) J Anal At Spectrom 17:1284–1289CrossRefGoogle Scholar
  14. 14.
    Ruzicka J, Marshall GD (1990) Anal Chim Acta 237:329–343CrossRefGoogle Scholar
  15. 15.
    Paseková H, Polášek M (2000) Talanta 52:67–75CrossRefGoogle Scholar
  16. 16.
    Wang AJ, Fan J, Feng SL, Cui FL (2005) Spectrosc Spec Anal 25(3):432–435Google Scholar
  17. 17.
    Stein S, Bohlen P, Udenfriend S (1974) Arch Biochem Biophys 163:400–403CrossRefPubMedGoogle Scholar
  18. 18.
    Lin DJ, Li LD (1997) Spectrosc Spec Anal 17(3):10–15Google Scholar
  19. 19.
    De Silva JAF, Strojny N (1975) Anal Chem 47:714–718CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  1. 1.Research Centre for Analytical SciencesNortheastern UniversityShenyangP.R. China

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