Analytical and Bioanalytical Chemistry

, Volume 385, Issue 4, pp 753–759 | Cite as

Multivariate optimisation of the microwave-assisted extraction of oleuropein and related biophenols from olive leaves

  • R. Japón-Luján
  • J. M. Luque-Rodríguez
  • M. D. Luque de CastroEmail author
Original Paper


Microwave assistance is proposed for the first time in order to accelerate the extraction of biophenols from olive leaves. Under optimal working conditions, obtained using a multivariate methodology, complete extraction of the target analytes was achieved in 8 min. The extracts required no clean-up nor concentration prior to injection into a chromatograph–photodiode array detector assembly for individual separation–quantification. The optimal extractant (an 80:20 ethanol–water mixture) was also used in the development of a stirring-based extraction method which required around 24 h for complete extraction of the target compounds. These mixtures can be used as replacements for toxic extractants, with a view to exploiting olive leaves in order to obtain biophenols for human use.


Microwave-assisted extraction Oleuropein Biophenols Polyphenols OBPs Olive leaves 



The Spanish Comisión Interministerial de Ciencia y Tecnología (CICyT) is gratefully acknowledged for financial support (project No. BQU-2002-13339).


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Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • R. Japón-Luján
    • 1
  • J. M. Luque-Rodríguez
    • 1
  • M. D. Luque de Castro
    • 1
    Email author
  1. 1.Analytical Chemistry Department, Annex Marie Curie Building, Rabanales CampusUniversity of CórdobaCórdobaSpain

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