Analytical and Bioanalytical Chemistry

, Volume 385, Issue 4, pp 780–786 | Cite as

Selective solid-phase extraction of bisphenol A using molecularly imprinted polymers and its application to biological and environmental samples

  • Jiang-hua Zhang
  • Ming Jiang
  • Lijun Zou
  • Dan Shi
  • Su-rong Mei
  • Ye-xiang Zhu
  • Yun Shi
  • Kang Dai
  • Bin LuEmail author
Original Paper


Molecularly imprinted polymers (MIPs) were prepared using bisphenol A (BPA) as a template by precipitation polymerization. The polymer that had the highest binding selectivity and ability was used as solid-phase extraction (SPE) sorbents for direct extraction of BPA from different biological and environmental samples (human serum, pig urine, tap water and shrimp). The extraction protocol was optimized and the optimum conditions were as follows: conditioning with 5 mL methanol–acetic acid (3:1), 5 mL methanol, 5 mL acetonitrile and 5 mL water, respectively, loading with 5 mL aqueous samples, washing with 1 mL acetonitrile, and eluting with 3 mL methanol. MIPs can selectively recognize, effectively trap and preconcentrate BPA over a concentration range of 2–20 μM. Recoveries ranged from 94.03 to 105.3 %, with a relative standard deviation lower than 7.9 %. Under the optimal condition, molecularly imprinted SPE recoveries of spiked human serum, pig urine, tap water and shrimp were 65.80, 82.32, 76.00 and 75.97 %, respectively, when aqueous samples were applied directly. Compared with C18 SPE, a better baseline, better high-performance liquid chromatography separation efficiency and higher recoveries were achieved after molecularly imprinted SPE.


Molecularly imprinted polymers Solid-phase extraction Bisphenol A Biological and environmental samples 



This work was supported by the National Natural Science Foundation of China (grant nos. 30200061 and 20477013).


  1. 1.
    Haighton LA, Hlywka JJ, Doull J, Kroes R, Lynch BS, Munro IC (2002) Regul Toxicol Pharmacol 35:238–254CrossRefGoogle Scholar
  2. 2.
    Xu J, Osuga Y, Yano T, Morita Y, Tang XH, Fujiwara T, Takai Y, Matsumi H, Koga K, Taketani Y, Tsutsumi O (2002) Biochem Biophys Rese Commun 292:456–462CrossRefGoogle Scholar
  3. 3.
    Safe SH, Pallaroni L, Yoon K, Gaido K, Ross S, McDonnell D (2002) Environ Health Perspect 110:925–929Google Scholar
  4. 4.
    Sajiki J (2001) J Chromatogr B 755:9–15CrossRefGoogle Scholar
  5. 5.
    Sajiki J, Takahashi K, Yonekubo J (1999) J Chromatogr B 736:255–261CrossRefGoogle Scholar
  6. 6.
    Ouchi K, Watanabe S (2002) J Chromatogr B 780:365–370CrossRefGoogle Scholar
  7. 7.
    Inoue K, Kawaguchi M, Funakoshi Y, Nakazawa H (2003) J Chromatogr B 798:17–23CrossRefGoogle Scholar
  8. 8.
    Salafranca J, Batlle R, Nerýn C (1999) J Chromatogr A 864:137–144CrossRefGoogle Scholar
  9. 9.
    Mori M, Naraoka H, Tsue H, Morozumi T, Kaneta T, Tanaka S (2001) Anal Sci 17:763–768CrossRefGoogle Scholar
  10. 10.
    Osemwengie LI, Steinberg S (2001) J Chromatogr A 932:107–118CrossRefGoogle Scholar
  11. 11.
    Pyrzynska K (2003) Chem Anal 48:781–795Google Scholar
  12. 12.
    Hennion MC, Pichon V (2003) J Chromatogr A 1000:29–52CrossRefGoogle Scholar
  13. 13.
    Delaunay N, Pichon V, Hennion MC (2000) J Chromatogr B 745:15–37CrossRefGoogle Scholar
  14. 14.
    Rashid BA, Aherne GW, Katmeh MF, Kwasowski P, Stevenson D (1998) J Chromatogr A 797:245–250CrossRefGoogle Scholar
  15. 15.
    Sellergren B (1994) Anal Chem 66:1578–1582CrossRefGoogle Scholar
  16. 16.
    Martin P, Wilson ID, Morgan DE, Jone GR, Jones K (1997) Anal Commun 34:45–47CrossRefGoogle Scholar
  17. 17.
    Rashid BA, Briggs RJ, Hay JN, Stevenson D (1997) Anal Commun 34:303–305CrossRefGoogle Scholar
  18. 18.
    Theodoridis G, Manesiotis P (2002) J Chromatogr A 948:163–169CrossRefGoogle Scholar
  19. 19.
    Wlashe M, Howarth J, Kelly MT, O’Kennedy R, Smyth MR (1997) J Pharm Biomed Anal 16:319–325CrossRefGoogle Scholar
  20. 20.
    Zander A, Findlay P, Renner T, Sellergren B (1998) Anal Chem 70:3304–3314CrossRefGoogle Scholar
  21. 21.
    Crescenzi C, Bayoudh S, Cormack PAG, Klein T, Ensing K (2001) Anal Chem 73:2171–2177CrossRefGoogle Scholar
  22. 22.
    Kawaguchi M, Hayatsu Y, Nakata H, Ishii Y, Ito R, Saito K, Nakazawa H (2005) Anal Chim Acta 539:83–89CrossRefGoogle Scholar
  23. 23.
    Sanbe H, Haginaka J (2002) J Pharm Biomed Anal 30:1835–1844CrossRefGoogle Scholar
  24. 24.
    Sambe H, Hoshina K, Hosoya K (2005) Analyst 130:38–40CrossRefGoogle Scholar
  25. 25.
    Li K, Stover HDH (1993) J Polym Sci Part A Polym Chem 31:3257–3263CrossRefGoogle Scholar
  26. 26.
    Ye L, Cormack PAG, Mosbach K (2001) Anal Chim Acta 435:187–196CrossRefGoogle Scholar
  27. 27.
    Whitcombe MJ, Rodriguez ME, Villar P, Vulfson EN (1995) J Am Chem Soc 117:7105–7111CrossRefGoogle Scholar
  28. 28.
    Hishiya T, Shibata M, Kakazu M, Asanuma H, Komiyama M (1999) Macromolecules 32:2265–2269CrossRefGoogle Scholar
  29. 29.
    Inoue K, Kato K, Yoshimura Y, Makino T, Nakazawa H (2000) J Chromatogr B 749:17–23CrossRefGoogle Scholar
  30. 30.
    Lubke C, Lubke M, Whitcombe MJ, Vulfson EN (2000) Macromolecules 33:5098–5105CrossRefGoogle Scholar
  31. 31.
    Umpleby II RJ, Rushton GT, Shah RN, Rampey AM, Bradshaw JC, Berch JK, Shimizu KD (2001) Macromolecules 34:8446–8452CrossRefGoogle Scholar
  32. 32.
    Chow CF, Lam MHW, Leung MKP (2002) Anal Chim Acta 466:17–30CrossRefGoogle Scholar
  33. 33.
    Vaidya AA, Lele BS, Kulkarni MG, Mashelkar RA (2001) J Appl Polym Sci 81:1075–1083CrossRefGoogle Scholar
  34. 34.
    Andersson LI (2000) Analyst 125:1515–1517CrossRefGoogle Scholar
  35. 35.
    Ikegami T, Mukawa T, Nariai H, Takeuchi T (2004) Anal Chim Acta 504:131–135CrossRefGoogle Scholar
  36. 36.
    Muldoon MT, Stanker LH (1997) Anal Chem 69:803–808CrossRefGoogle Scholar
  37. 37.
    Molinelli A, Weiss R, Mizaikoff B (2002) J Agric Food Chem 50:1804–1808CrossRefGoogle Scholar
  38. 38.
    Moller K, Crescenzi C, Nilsson U (2004) Anal Bioanal Chem 378:197–204CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Jiang-hua Zhang
    • 1
  • Ming Jiang
    • 1
  • Lijun Zou
    • 1
  • Dan Shi
    • 1
  • Su-rong Mei
    • 1
  • Ye-xiang Zhu
    • 1
  • Yun Shi
    • 1
  • Kang Dai
    • 1
  • Bin Lu
    • 1
    Email author
  1. 1.MOE Key Laboratory of Environment & Health, Institute of Environmental Medicine, School of Public Health, Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanP.R. China

Personalised recommendations