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Study of the bioavailability of selenium in cows’ milk after a supplementation of cow feed with different forms of selenium


The purpose of the work described in this paper was to develop an easy and quick in-vitro method for comparing the bioavailability of selenium in cows’ milk after different cow feed. The study focuses on bioavailability differences resulting from the use of different selenium species (organic selenium as selenised yeast and sodium selenite) for supplementation of forage. A procedure for determination of selenium in cows’ milk and dialysates, by hydride-generation atomic-fluorescence spectrometry (HG-AFS) after microwave-assisted acid digestion, was optimised. The results show it is possible to obtain cows’ milk enriched with selenium at different concentration without altering the original composition of the milk. The bioavailability was statistically greater for cows’ milk obtained after supplementation of forage with organic selenium at levels of 0.4 and 0.5 μg Se g−1 than for that obtained after supplementation with inorganic and organic selenium at levels of 0.2 and 0.3 μg Se g−1.

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The authors gratefully acknowledge support of this work by FEIRACO Sociedad Cooperativa Gallega.

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Correspondence to Pilar Bermejo-Barrera.

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Muñiz-Naveiro, Ó., Domínguez-González, R., Bermejo-Barrera, A. et al. Study of the bioavailability of selenium in cows’ milk after a supplementation of cow feed with different forms of selenium. Anal Bioanal Chem 385, 189–196 (2006).

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  • Selenium
  • Cows’ milk
  • In-vitro digestion
  • Selenium bioavailability
  • Hydride-generation atomic-fluorescence spectrometry