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Renewable stationary phase liquid magnetochromatography: determining aspartame and its hydrolysis products in diet soft drinks

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Abstract

A new chromatographic modality that does not require high pressures and also allows renewal of the stationary phase as desired is reported. The technique is based on a thin layer paramagnetic stationary phase (Fe3O4–SiO2) retained on the inner wall of a minicolumn through the action of an external magnetic field, which also plays an important role in separating the analytes. Accordingly, the name “renewable stationary phase liquid magnetochromatography”, or RSP-LMC, has been proposed for it. The technique was used to separate and quantify the sugar substitute α-aspartame and its constituent amino acids (hydrolysis products), L-aspartic acid and L-phenylalanine, in diet fizzy soft drinks. When the results obtained for α-aspartame were compared with those obtained using HPLC as a reference method, no significant differences were observed. The system proposed is fully automated, making it an economic, competitive alternative to conventional methods of determining α-aspartame and its amino acid components.

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Acknowledgements

This work was supported by the Dirección General de Investigación of the MCyT-FEDER, project BQU2003/03481 and the Junta de Castilla y León (VA067/04). J.A. Rodriguez was awarded a grant by the CONACyT.

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Authors and Affiliations

  1. Departamento de Química Analítica, Facultad de Ciencias, Universidad de Valladolid, Paseo Prado de la Magdalena s/n, 47005, Valladolid, Spain

    E. Barrado, J. A. Rodríguez & Y. Castrillejo

  2. Centro de Investigaciones Químicas, Universidad Autónoma del Estado de Hidalgo, Ctra. Pachuca-Tulancingo Km 4.5, 42076, Pachuca, Hidalgo, Mexico

    J. A. Rodríguez

Authors
  1. E. Barrado
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  2. J. A. Rodríguez
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  3. Y. Castrillejo
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Correspondence to E. Barrado.

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Barrado, E., Rodríguez, J.A. & Castrillejo, Y. Renewable stationary phase liquid magnetochromatography: determining aspartame and its hydrolysis products in diet soft drinks. Anal Bioanal Chem 385, 1233–1240 (2006). https://doi.org/10.1007/s00216-006-0356-y

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  • DOI: https://doi.org/10.1007/s00216-006-0356-y

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