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Reflectometric interference spectroscopy combined with MALDI−TOF mass spectrometry to determine quantitative and qualitative binding of mixtures of vancomycin derivatives

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Abstract

This paper describes the combination of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry with label free bio-interaction analysis based on reflectometric interference spectroscopy (RIfS). The potential of this concerted approach is demonstrated by measuring the binding properties of different vancomycin-type glycopeptide antibiotic mixtures. Although RIfS is sensitive and does not require use of a label, it cannot determine which components of a mixture have bound to the surface after incubation. Fortunately, each bound species has a unique mass that can, afterwards, be determined by mass spectrometry. Thus, the screening capability of RIfS is combined with the identification capability of mass spectrometry.

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Acknowledgments

Martin Mehlmann was supported by the DFG-Graduate colleague “Quantitative Analysis and Characterisation of Pharmaceutically and Biochemically relevant Substances” at the University of Tübingen and by the Fonds der Chemischen Industrie. Desvancosamin-vancomycin was a kind gift from Dr Evi Stegmann, AG Wohlleben, University of Tübingen.

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Author notes

  1. Martin Mehlmann

    Present address: Department of Chemistry and Biochemistry, University of Colorado, 80309, Boulder, CO, USA

Authors and Affiliations

  1. Institute for Physical and Theoretical Chemistry, University of Tübingen, 72074, Tuebingen, Germany

    Martin Mehlmann & Günter Gauglitz

  2. Applied Biosystems, Applera Deutschland GmbH, Frankfurter Str. 129b, 64293, Darmstadt, Germany

    Alex M. Garvin & Michael Steinwand

Authors
  1. Martin Mehlmann
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  2. Alex M. Garvin
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  3. Michael Steinwand
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  4. Günter Gauglitz
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Correspondence to Günter Gauglitz.

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Dedicated to the memory of Wilhelm Fresenius

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Mehlmann, M., Garvin, A.M., Steinwand, M. et al. Reflectometric interference spectroscopy combined with MALDI−TOF mass spectrometry to determine quantitative and qualitative binding of mixtures of vancomycin derivatives. Anal Bioanal Chem 382, 1942–1948 (2005). https://doi.org/10.1007/s00216-005-3329-7

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  • DOI: https://doi.org/10.1007/s00216-005-3329-7

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