Skip to main content
SpringerLink
Log in

Chemiluminescence immunoassay for chloramphenicol

  • Original Paper
  • Published:
Analytical and Bioanalytical Chemistry Aims and scope Submit manuscript

Abstract

In recent years, various chemiluminescent clinical immunoassay kits have been widely applied to the detection of hormones. However, a kit for chloramphenicol (CAP) is often absent from most commercial product lists, even though it is important to control the levels of CAP residues in foodstuffs too. Therefore, we describe a simple, solid-phase chemiluminescence immunoassay (CLIA) for the measurement of CAP in foodstuffs. A rabbit anti-CAP IgG is passively adsorbed onto the walls of polypropylene plates. The labeled antigen is horseradish peroxidase (HRP) conjugate of CAP. Luminol solution is used as the substrate of HRP. The light yield is inversely proportional to the concentration of CAP. The method has a similar sensitivity (0.05 ng/ml), specificity, precision, and accuracy to a conventional enzyme immunoassay (EIA). The intra-assay and inter-assay CVs of ten samples were <8% and <20%, respectively, and the analytical recovery of the method was 87–100%. The experimental correlation coefficient of dilution was found to be 0.999 using milk supernatant as buffer. The detection limit for the method was 0.1–10 ng/ml, and it displayed good linearity.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  1. Ohno K-I, Arakawa H (1999) Development of novel high-sensitivity chemiluminescence assay for luminol using thiourea derivatives. Luminescence 14:355–360

    Article  CAS  PubMed  Google Scholar 

  2. Whitehead TP, Kricka LJ, Carter TJN, Thorpe GHG (1979) Analytical luminescence: its potential in the clinical laboratory. Clin Chem 25(9):1531–1546

    CAS  PubMed  Google Scholar 

  3. Kim JB, Barnard GJ, Collins WP, Kohen F, Lindner HR, Eshhar Z (1982) Measurement of plasma estradiol-17β by solid-phase chemiluminescence immunoassay. Clin Chem 28(5):1120–1124

    Google Scholar 

  4. Javier Bagazgoitia F, Luis Garcia J, Ibarra JM, Patino R (1989) Chemiluminescent immunoassay (CLIA) for urinary albumin. J Biolumin Chemilum 3:169–174

    Google Scholar 

  5. Hubl W, Thorpe GHG, Hofmann F, Meissner D, Thiele HJ (1990) Enhanced cheniluminescent immunoassay for aldosterone. J Biolumin Chemilumin 5:49–52

    Article  CAS  PubMed  Google Scholar 

  6. Aoyagi S, Iwata T, Miyasaka T, Sakai K (2001) Determination of human serum albumin by chemiluminescence immunoassay with luminol using a platinum-immobilized flow-cell. Analytica Chimica Acta 436:103–108

    Article  CAS  Google Scholar 

  7. Thorpe GHG, Kricka LJ (1986) Enhanced chemiluminescent reactions catalyzed by horseradish peroxidase. Methods Enzymol 133:331–353

    CAS  PubMed  Google Scholar 

  8. Kricka LJ, Ji X (1995) 4-Phenylylboronic acid: a new type of enhancer for the horseradish peroxidase catalyzed chemiluminescent oxidation of luminol. J Biolumin Chemilumin 10:49–54

    Article  CAS  PubMed  Google Scholar 

  9. Kricka LJ, Cooper M, Ji X (1996) Synthesis and characterization of 4-iodophenylboronic acid: a new enhancer for the horseradish peroxidase-catalyzed chemiluminescent oxidation of luminol. Anal Biochem 240:119–125

    Article  CAS  PubMed  Google Scholar 

  10. Lynas L (1998) Screening for chloramphenicol residues in the tissues and fluids of treated cattle by the four-plate test, Charm II radioimmunoassay and Ridascreen CAP-Glucuronid enzyme immunoassay. Analyst 123:2773–2777

    Article  CAS  PubMed  Google Scholar 

  11. Liu Z-h (1995) Enzyme immunoassay for assaying chloramphenicol in animal foodstuff. Chin J Vet Drug 29(2):47–48

    Google Scholar 

  12. Dzgoev AB (1999) High-sensitivity assay for pesticide using a peroxidase as chemiluminescent label. Anal Chem 71:5258–5261

    Article  CAS  PubMed  Google Scholar 

  13. Wang C-J (1993) A sensitive enzyme immunoassay (EIA) for quantitation of the topical anti-inflammatory agent SCH 40120 in unectracted human plasma. J Pharm Biomed Anal 11(10):871–879

    Article  CAS  PubMed  Google Scholar 

  14. Xu CL (2003) A determination of chloramphenicol in animal foodstuff with enzyme immunoassay[P]. CN1389729A, 2003-1–8

  15. Zhou DG, Fang H (2003) The evaluation of three kinds of chloramphenicol-ELISA kits. Chin J Vet Drug 37(9):28–31

    CAS  Google Scholar 

  16. Wang J (2002) A high stability chemiluminescence substrate and its determination[P]. CN1333464A, 2002-1–30

  17. Iwata R, Ito H, Hayashi T, Sekine Y, Koyama N, Yamaki M (1995) Stable and general-purpose chemiluminescent detection system for horseradish peroxidase employing a thiazole compound enhancer and some additives. Anal Biochem 231:170–174

    Article  CAS  PubMed  Google Scholar 

  18. De-shi S, Bin Z, Ya-li Q, Gui-zhi W (2002) Establishment of indirect competitive enzyme-linked immunosorbent assay for detecting the residues of CAP. Chin J Vet Sci 22(1):77–79

    Google Scholar 

  19. Meifang S, Wenya Z, Zhiliang F, Guanghong W, Xuebing G (2003) Enzyme linked immunosorbent assay (ELISA) for quantitative analysis of chloramphenicol residues in aquatic products. J Nanjing Normal Univ (Eng Technol) 3(2):1–4

    Google Scholar 

Download references

Conflict of interest:

No information supplied

Author information

Authors and Affiliations

  1. Isotope Department, China Institute of Atomic Energy, Beijing, 102413, R China

    Si Lin, Shi-quan Han, Yi-bing Liu, Wen-ge Xu & Guo-ying Guan

Authors
  1. Si Lin
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Shi-quan Han
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yi-bing Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Wen-ge Xu
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Guo-ying Guan
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Si Lin.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Lin, S., Han, Sq., Liu, Yb. et al. Chemiluminescence immunoassay for chloramphenicol. Anal Bioanal Chem 382, 1250–1255 (2005). https://doi.org/10.1007/s00216-005-3273-6

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00216-005-3273-6

Keywords

Search

Navigation