Abstract
An HPLC method for determination of rofecoxib in human serum is presented. The method is based on pre-column derivatization of analyte to a phenanthrene derivative of the drug. Rofecoxib and the internal standard were extracted from serum using liquid–liquid extraction. Upon exposure to UV light, the drug was found to undergo a photocyclization reaction, giving a species with high absorbance. Validation of the method has been studied in the concentration range 10–500 ng ml−1.
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Acknowledgements
This work was supported by grants from the Research Council of Tehran University of Medical Sciences and the Iran Chapter of TWAS.
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Amini, M., Hamedani, M.P., Vosooghi, M. et al. Pre-column derivatization of rofecoxib for determination in serum by HPLC. Anal Bioanal Chem 382, 1265–1268 (2005). https://doi.org/10.1007/s00216-005-3249-6
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DOI: https://doi.org/10.1007/s00216-005-3249-6