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The selectivity in MEKC of pseudo-stationary phases based on polyelectrolyte complexes: I. composition of the complex

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Abstract

Polyelectrolyte–surfactant complexes (PSC) of polycarboxylic acids with alkyl-trimethylammonium salts look very promising as a new type of pseudo-stationary phase in micellar electrokinetic chromatography. PSC produce an intramolecular micellar phase, and the morphology of the micelles is significantly different from that of the corresponding “typical” surfactant micelles. Pseudo-stationary phases based on PSC have unique selectivity. In this paper, the effect of the composition (φ) of the PSC of polyacrylic acid (PAA) MW 130,000 with dodecyltrimethylammonium bromide (DTAB) and of the PSC of PAA MW 450,000 with DTAB on the separation of DNS–amino acids and phenol derivatives in these systems was investigated. Relative retention and relative selectivity were used to describe the electrophoretic behavior of the amino acids and phenol derivatives. The main advantage of PSC pseudo-phases is that the nature and the structure of micelle-like units, and hence the selectivity of electrophoretic separation, could easily be modified by changing the composition of the complex.

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References

  1. Palmer CP, Tanaka N (1997) J Chromatogr A 792:105–124

    Article  CAS  Google Scholar 

  2. Palmer CP (1997) J Chromatogr A 780:75–92

    Article  CAS  Google Scholar 

  3. Shpak AV, Pirogov AV, Shpigun OA (2004) J Chromatogr B 800:91–100

    Article  CAS  Google Scholar 

  4. Leonard MS, Khaledi MG (2002) Electrophoresis 25:1019–1029

    CAS  Google Scholar 

  5. Zakaria P, Macka M, Fritz JS, Haddad PR (2002) Electrophoresis 23:2821–2832

    Article  CAS  PubMed  Google Scholar 

  6. Maichel B, Kenndler E (2000) Electrophoresis 21:3160–3173

    Article  CAS  PubMed  Google Scholar 

  7. Shamsi SA, Palmer CP, Warner IM (2001) Anal Chem 73:140a–149a

    Article  CAS  PubMed  Google Scholar 

  8. Palmer CP (2002) Electrophoresis 23:3993–4004

    Article  CAS  PubMed  Google Scholar 

  9. Goddard ED (1986) Colloid Surf 19:301–309

    Article  CAS  Google Scholar 

  10. Zakharova YuA, Kolbanovskiy AD, Krinitskaya LA, Kasaikin VA, Wasserman AM (1995) Polym Sci Ser B 37:439–443

    Google Scholar 

  11. Kasaikin VA, Litmanovich EA, Zezin AB, Kabanov VA (1999) Doklady Akad Nauk (in Russian) 367:359–362

    CAS  Google Scholar 

  12. Pirogov AV, Shpak AV, Shpigun OA (2003) Anal Bioanal Chem 375:1199–1203

    CAS  PubMed  Google Scholar 

  13. Kasaikin VA, Zakharova JA (1999) Colloids Surf A: Physicochem Eng Aspects 147:107–114

    Article  CAS  Google Scholar 

  14. Ibragimova ZKh, Ivleva YeM, Pavlova NV, Borodulina TA, Yefremov VA, Kasaikin VA, Zezin AB, Kabanov VA (1992) Vysokomolekyl Soed 34(A) 9:139–147

    Google Scholar 

Download references

Acknowledgements

This work was performed with financial support from the Russian Foundation for Basic Research, grant No. 04-03-32866. We are grateful to the Division of High Molecular Compounds of Moscow State University and personally to Dr Yu.A. Zakharova for providing samples of the polyacrylic acids.

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Authors and Affiliations

  1. Chemistry Department, Moscow State University, GSP-2, Vorob‘evy Hills, 119992, Moscow, Russia

    Alexey V. Shpak, Andrey V. Pirogov & Oleg A. Shpigun

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  1. Alexey V. Shpak
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  2. Andrey V. Pirogov
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  3. Oleg A. Shpigun
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Correspondence to Alexey V. Shpak.

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Shpak, A.V., Pirogov, A.V. & Shpigun, O.A. The selectivity in MEKC of pseudo-stationary phases based on polyelectrolyte complexes: I. composition of the complex. Anal Bioanal Chem 382, 504–512 (2005). https://doi.org/10.1007/s00216-005-3219-z

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  • DOI: https://doi.org/10.1007/s00216-005-3219-z

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