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A microwave-assisted microassay for lipases

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A quick and efficient two-step assay for monitoring and screening lipase activity that uses a microtitre plate is described.

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  1. Bosley JA, Peilow AD (2000) In: Gupta MN (ed) Methods in non-aqueous enzymology. Birkhauser, Basel, pp 52–69

  2. Carrea G, Riva S (2000) Angew Chem Int Edit 39:2226–2254

    Google Scholar 

  3. Roy I, Gupta MN (2004) Eur J Biochem 271:1–9

    Google Scholar 

  4. Beisson F, Tiss A, Riviere C, Verger R (2000) Eur J Lipid Sci Technol 28:133–153

    Google Scholar 

  5. Kilcawley KN, Wilkinson MG, Fox PF (2002) Enzyme Microb Tech 31:310–320

    Google Scholar 

  6. Pencreac’h G, Barratti JC (1996) Enzyme Microb Tech 18:417–422

    Google Scholar 

  7. Gupta N, Rathi P, Gupta R (2002) Anal Biochem 311:98–99

    Google Scholar 

  8. Devec C, Rodriguez-Lopez JN, Fenoll LG, Tudela J, Catala JM, Reyes ED, Garcia-Canovas F (1999) J Agr Food Chem 47:4506–4511

    Google Scholar 

  9. Roy I, Gupta MN (2003) Curr Sci 85:1685–1693

    Google Scholar 

  10. Chen ST, Sookkheo B, Phutrahul S, Wang KT (2001) In: Vulfson EN, Halling PJ, Holland HL (eds) Enzymes in nonaqueous solvents: methods and protocols. Humana, Totowas, NJ, pp 373–400

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This work was supported by project funds from Department of Science and Technology (Government of India). Financial support provided by CSIR to SJ (SRF) and UGC to PJ (JRF) is also gratefully acknowledged.

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Correspondence to M. N. Gupta.

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Jain, P., Jain, S. & Gupta, M.N. A microwave-assisted microassay for lipases. Anal Bioanal Chem 381, 1480–1482 (2005).

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