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A kinetic model and its parameter estimation for the process of binding copper to human serum albumin by a voltammetric method

Analytical and Bioanalytical Chemistry volume 381, pages 1552–1557 (2005)Cite this article

Abstract

Linear sweep anodic stripping voltammetry was applied to determine the concentration of free copper ions in the process of binding copper to human serum albumin (HSA) on the mercaptoethane sulfonate modified gold electrode surface. A kinetic model of two consecutive steps for the process of binding copper to HSA was first proposed on the basis of the electrochemical results and compared with a parallel kinetic response model by using residual analysis. The experimental data of the stripping peak currents with time was fitted according to the model and the kinetic parameters, binding rate constants, k1 and k2, were estimated to be 0.411 and 0.055 min−1, respectively.

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Acknowledgements

This work was supported by Doctorate Fund of the Education Department of China (No. 20020532007) and Key Sci/Tech Research Project of Education Department of China (No. 03123).

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  1. State Key Laboratory of Chem/Biosensing and Chemometrics College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, P.R. China

    Kejun Zhong, Jianjun Xia, Wanzhi Wei, Yanbo Hu, Han Tao & Wei Liu

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  1. Kejun Zhong
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  2. Jianjun Xia
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  3. Wanzhi Wei
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Correspondence to Wanzhi Wei.

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Zhong, K., Xia, J., Wei, W. et al. A kinetic model and its parameter estimation for the process of binding copper to human serum albumin by a voltammetric method. Anal Bioanal Chem 381, 1552–1557 (2005). https://doi.org/10.1007/s00216-005-3104-9

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  • DOI: https://doi.org/10.1007/s00216-005-3104-9

Keywords

  • Kinetic model
  • Binding process
  • Copper
  • Human serum albumin
  • Linear sweep anodic stripping voltammetry (LSASV)
  • Mercaptoethane sulfonate (MES)