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Flow injection chemiluminescence immunoassay for 17β-estradiol using an immunoaffinity column

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Abstract

A new flow injection chemiluminescent immunoassay was developed for the detection of 17β-estradiol (E2). The method uses p–iodophenol (PIP) as enhancer and is based on a solid-phase immunoassay format in which an E2–OVA immobilized immunoaffinity column inserted in the flow system is used to trap unbound horseradish peroxidase (HRP)-labeled anti-E2 antibody after an off-line incubation of E2 with HRP-labeled anti-E2 antibody. The trapped enzyme conjugate was detected by injecting substrates to produce an enhanced chemiluminescence (CL) response. The linear range for E2 was 10.0–1,000.0 ng mL−1 with a correlation coefficient of 0.996 and a detection limit of 3.0 ng mL−1. The sampling and chemiluminescence detection time for one sample was 400 s after a pre-incubation procedure of 30 min. Serum samples detected by this method were in good agreement with the results obtained by EIA with E2–biotin.

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References

  1. McLachlan J, Arnold S (1996) Am Sci 84:452

    ADS  Google Scholar 

  2. Colborn T, Saal F, Soto A (1993) Environ Health Perspect 101:378

    Article  PubMed  CAS  Google Scholar 

  3. Aherne GW, English J, Marks V (1985) Ecotoxicol Environ Saf 9:361

    Article  Google Scholar 

  4. Shore LS, Gurevitz M, Shemesh M (1993) Bull Environ Contam Toxicol 51:361

    Article  PubMed  CAS  Google Scholar 

  5. Valentini F, Compagnone D, Gentili A, Palleschi G (2002) The Analyst 127:1333–1337

    Article  PubMed  CAS  Google Scholar 

  6. Desbrow C, Routledge EJ, Brighty GC, Sumpter JP, Valdok M (1998) Environ Sci Technol 32:1549

    Article  CAS  Google Scholar 

  7. Belfroid AC, Horst AV, Vethaak AD, Schafer AJ, Rijs GBJ, Wegener J, Cofino WP (1999) Sci Total Environ 225:101

    Article  PubMed  CAS  Google Scholar 

  8. Ternes TA, Stumpf M, Mueller J, Haberer K, Wilken RD, Servos M (1999) Sci Total Environ 225:81

    Article  PubMed  CAS  Google Scholar 

  9. Kuch HM, Ballschmiter K (2000) Fresenius J Anal Chem 366:392

    Article  PubMed  CAS  Google Scholar 

  10. Ishii Y, Okita S, Torigai M, Yun SJ (2000) Bunseki Kagaku 49:758

    Google Scholar 

  11. Jabri JE (1991) J Steroid Biochem Mol Biol 38:339

    Article  PubMed  Google Scholar 

  12. Bourve J, Boever JD, Leyseele D, Bosmans E, Dubois P, Kohen F, Vandekerchhove D (1992) Clin Chem 38:1409

    Google Scholar 

  13. Lixia Z, Jin–Ming L (2005) J Biotechnol 118:177

    Article  PubMed  CAS  Google Scholar 

  14. Sato H, Mochizuki H, Tomita Y, Kanamori T (1996) Clin Biochem 29:509

    Article  PubMed  CAS  Google Scholar 

  15. De Boever J, Kohen F, Bouve J, Leyseele D, VandeKerckhove D (1990) Clin Chem 36:2036

    PubMed  Google Scholar 

  16. Sankolli GM, Stott RA, Thorpe GH, Smith D, Rudd BT, Kricka LJ (1988) Ann Clin Biochem 25(Pt 3):288

    PubMed  CAS  Google Scholar 

  17. De Boever J, Kohen F, Usanachitt C, Vandekerckhove D, Leyseele D, Vandewalle L (1986) Clin Chem 32:1895

    PubMed  Google Scholar 

  18. Roda A, Girotti S, Piacentini AL, Preti S, Lodi S (1986) Anal Biochem 156:267

    Article  PubMed  CAS  Google Scholar 

  19. Ruzicka J, Hansen EH (1975) Anal Chim Acta 78:145

    Article  CAS  Google Scholar 

  20. Dietrich M, Krämer PM (1995) Food Agric Immunol 7:203

    Article  CAS  Google Scholar 

  21. Krämer PM, Baumann BA, Stoks PG (1997) Anal Chim Acta 347:187

    Article  Google Scholar 

  22. González–Martineez MA, Morais S, Puchades R, Maquieira A, Marco PM, Barcelü D (1998) Fresenius J Anal Chem 361:179

    Article  Google Scholar 

  23. Pollema CH, Ruzicka J (1994) Anal Chem 66:1825

    Article  PubMed  CAS  Google Scholar 

  24. Mayer M, Ruzicka J (1996) Anal Chem 68:3808

    Article  CAS  Google Scholar 

  25. Gunaratna PC, Wilson GS (1993) Anal Chem 65:1152–1157

    Article  PubMed  CAS  Google Scholar 

  26. Dhar TK, Samanta AK, Ali E (1988) Steroids 51:519

    Article  PubMed  CAS  Google Scholar 

  27. Langone JJ, Vunakis HV (1975) Res Commun Pathol Pharmacol 10:163

    CAS  Google Scholar 

  28. Tang D, Wang YC, Chang WB, Ci YX, Guo ZQ (1999) Chin J Anal Chem 27:899

    CAS  Google Scholar 

  29. Liu HJ, Yu JC, Bindra DS, Givens RS, Wilson GS (1991) Anal Chem 63:666

    Article  CAS  Google Scholar 

  30. Lin JH, Yan F, Ju HX (2004) Clin Chim Acta 341:109

    Article  PubMed  CAS  Google Scholar 

  31. Zhao JF, Wang YC, Li YZ, Chang WB (2004) Chin J Chem 22:708

    Article  CAS  Google Scholar 

Download references

Acknowledgement

We were grateful for the financial support of the National Natural Science Foundation of China (No. 20277005).

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Authors and Affiliations

  1. College of Environmental Science and Engineering, Donghua University, Shanghai, 200051, People’s Republic of China

    Shuhao Wang & Huisheng Zhuang

  2. Institute of Chemistry and Chemical Technology, Liaocheng University, Liaocheng, 252059, People’s Republic of China

    Shuhao Wang, Shilei Lin & Lingyun Du

Authors
  1. Shuhao Wang
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  2. Shilei Lin
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  3. Lingyun Du
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  4. Huisheng Zhuang
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Corresponding author

Correspondence to Huisheng Zhuang.

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Wang, S., Lin, S., Du, L. et al. Flow injection chemiluminescence immunoassay for 17β-estradiol using an immunoaffinity column. Anal Bioanal Chem 384, 1186–1190 (2006). https://doi.org/10.1007/s00216-005-0268-2

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  • DOI: https://doi.org/10.1007/s00216-005-0268-2

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