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An optical glucose biosensor based on glucose oxidase immobilized on a swim bladder membrane

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Abstract

An optical glucose biosensor using a swim bladder membrane as an enzyme immobilization platform and an oxygen-sensitive membrane as an optical oxygen transducer has been developed. During the enzymatic reaction, glucose is oxidized by glucose oxidase with a concomitant consumption of dissolved oxygen resulting in an increase in the fluorescence intensity of the optical oxygen transducer. The fluorescence intensity is directly related to the glucose concentration. The effects of pH, temperature, buffer concentration, and selectivity have been studied in detail. The immobilized enzyme retained 80% of its initial activity after being kept for more than 10 months at 4°C. The glucose biosensor has been successfully applied to the determination of glucose content in human blood serum and urine samples.

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Authors and Affiliations

  1. College of Chemistry, Sichuan University, Chengdu, 610065, P.R. China

    Zaide Zhou & Lina Qiao

  2. College of Chemical Engineering, Sichuan University, Chengdu, 610065, P.R. China

    Peng Zhang & Dan Xiao

  3. Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Hong Kong SAR, P.R. China

    Martin M. F. Choi

Authors
  1. Zaide Zhou
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  2. Lina Qiao
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  3. Peng Zhang
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  4. Dan Xiao
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  5. Martin M. F. Choi
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Corresponding authors

Correspondence to Dan Xiao or Martin M. F. Choi.

Additional information

Martin M.F. Choi was on sabbatical leave at The University of North Carolina at Chapel Hill from July 2004 to July 2005.

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Zhou, Z., Qiao, L., Zhang, P. et al. An optical glucose biosensor based on glucose oxidase immobilized on a swim bladder membrane. Anal Bioanal Chem 383, 673–679 (2005). https://doi.org/10.1007/s00216-005-0023-8

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  • DOI: https://doi.org/10.1007/s00216-005-0023-8

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