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The determination of total Se in urine and serum by graphite furnace atomic absorption spectrometry using Ir as permanent modifier and in situ oxidation for complete trimethylselenonium recovery

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Abstract

The present work evaluated the use of iridium (Ir) as permanent modifier for the determination of total selenium in urine and serum by graphite furnace atomic absorption spectrometry. Concerning urine, the presence of trimethylselenonium (TMSe+) was especially considered. Pyrolysis and atomization temperatures of 1,000 and 2,100°C, respectively, were used. For nondigested urine and serum samples, 0.2% v/v HNO3 and Triton X-100 were used as diluents, respectively, and the same initial platform Ir treatment was effective for up to 1,100 atomization cycles. Good precision [less than 5% relative standard deviation (RSD)] can be achieved with the proposed method. Low TMSe+ recovery was observed for nondigested urine samples. Thus, if this species is to be considered in urine analysis, a previous external mineralization step was found to be necessary. Alternatively, an in situ oxidation treatment was developed. Detection limits of 8, 10, and 7 μg l−1 were obtained after dilution, microwave-assisted digestion, and in situ oxidation procedures, respectively. The accuracy of the method was validated by the analysis of certified reference or commercial quality control materials and spiked samples.

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Acknowledgements

The authors are grateful to the Brazilian National Research Council for the financial support, to the Sergio Franco Laboratories for the body fluids, and to Dr. B. Welz (Federal University of Santa Catarina, Brazil) for the donation of the organo Se compounds.

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Authors and Affiliations

  1. Department of Chemistry, Pontifícia Universidade Católica do Rio de Janeiro, Rua Marquês de São Vicente, 225, Rio de Janeiro, 22453-900, Brazil

    Patricia Grinberg, Rodrigo Araújo Gonçalves & Reinaldo Calixto de Campos

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  1. Patricia Grinberg
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  2. Rodrigo Araújo Gonçalves
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  3. Reinaldo Calixto de Campos
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Correspondence to Reinaldo Calixto de Campos.

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Grinberg, P., Gonçalves, R.A. & de Campos, R.C. The determination of total Se in urine and serum by graphite furnace atomic absorption spectrometry using Ir as permanent modifier and in situ oxidation for complete trimethylselenonium recovery. Anal Bioanal Chem 383, 1044–1051 (2005). https://doi.org/10.1007/s00216-005-0014-9

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  • DOI: https://doi.org/10.1007/s00216-005-0014-9

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