Abstract
Near-infrared reflectance spectroscopy was applied to determine nitrogen (N), phosphorus (P) and calcium (Ca) content in leaf samples of 18 woody species. A total of 183 samples from mountain, riparian and dry areas from the Central–Western Iberian Peninsula were collected for this purpose. The wide intervals of variation observed in nutrient concentrations (6.6–45.0 g kg−1 for N, 0.24–2.97 g kg−1 for P, and 1.00–20.06 g kg−1 for Ca) were due to the great heterogeneity of the samples. To develop calibration equations, multiple linear regression, and partial least-squares regression (PLSR) were used. In both cases, three mathematical transformations of the data were applied: log1/R and first and second derivatives. The best calibration statistics were obtained using PLSR and derivative transformations (second derivative for N and first derivative for P and Ca). The following coefficients of multiple determination (R2) and standard errors of cross validation were obtained: 0.99 and 0.93 for N, 0.94 and 0.15 for P, and 0.95 and 0.88 for Ca. In the external validation the standard errors of prediction obtained were 0.76 (N), 0.11 (P) and 0.60 (Ca).
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Norris KH, Barnes RF, Moore JE, Shenk JS (1976) J Anim Sci 43:889–897
Shenk JS, Westerhaus MO, Hoover MR (1979) J Dairy Sci 62:807–812
Williams P, Norris KH (eds) (1987) Near-infrared technology in the agricultural and food industries. Am Assoc Cereal Chem, Minnesota
Marten GC, Shenk JS, Barton II FE (eds) (1989) Near infrared reflectance spectroscopy (NIRS): analysis of forage quality. Agriculture handbook no 643 (revised). USDA, Washington
García-Ciudad A, García-Criado B, Pérez Corona ME, Vázquez de Aldana BR, Ruano Ramos AM (1993) J Sci Food Agric 63:419–426
Ruano-Ramos A, García-Ciudad A, García-Criado B (1999) Anim Feed Sci Technol 77:331–343
Foley WJ, McIlwee A, Lawler I, Aragones L, Woolnough AP, Berding N (1998) Oecologia 116:293–305
Murray I (1993) In: Davies A, Baker RD, Grant SA, Laidlaw AS (eds) Sward measurement handbook. British Grassland Society, Reading Berks, pp 286–312
Paul C (1989) In: Paul C, Zimmer E (eds) Nahinfrarotspektroskopie für Industrie und Landwirtschaft, Braunschweig, FAL, vol 107, pp 61–70
Batten GD, Blakeney AB (1992) In: Hildrum KI, IsaKsson T, Naes T, Tandberg A (eds) Near infra-red spectroscopy—bridging the gap between data analysis and NIR applications. Ellis Horwood, Chichester, pp 185–190
Clark DH, Mayland HF, Lamb RC (1987) Agron J 79:485–490
Givens DI, Deaville ER (1999) Aust J Agric Res 50:1131–1145
Clark DH, Cary EE, Mayland HF (1989) Agron J 81:91–95
Vázquez de Aldana BR, García Criado B, García Ciudad A, Pérez Corona ME (1995) Commun Soil Sci Plant Anal 26:1383–1396
Morón A, Cozzolino D (2002) J Agric Sci 139:413–423
Batten GD, Ciavarella S, Blakeney AB (2003) In: 11th international conference on near infrared spectroscopy, abstracts book, Córdoba, p 5.15
Meuret M, Dardenne P, Biston R, Poty O (1993) J Near Infrared Spectrosc 1:45–54
Bolster KL, Martin ME, Aber JD (1996) Can J For Res 26:590–600
McIlwee AM, Lawler IR, Cork SJ, Foley WJ (2001) Oecologia 128:539–548
García Ciudad A, Fernández Santos B, Vázquez de Aldana BR, Zabalgogeazcoa I, Gutiérrez MY, García Criado B (2004) Commun Soil Sci Plant Anal 35:665–678
Flinn PC, Edwards NJ, Oldham CM, McNeill DM (1996) In: Davies AMC, Williams P (eds) Near infrared spectroscopy: the future waves. NIR Publications, Chichester, pp 576–580
Hallett RA, Hornbeck JW, Martin ME (1997) J Near Infrared Spectrosc 5:77–82
Gillon D, Houssard C, Joffre R (1999) Oecologia 118:173–182
Saiga G, Sasaki T, Nonaka K, Takahashi K, Watanabe M, Watanabe K (1989) J Japan Grassl Sci 35:228–233
De Boever JL, Eeckhout W, Boucque ChV (1994) Neth J Agric Sci 42:357–369
Mediavilla S, Escudero A (2003) Plant Ecol 168:321–332
Duque Macias F (1971) Anal Edafol Agrobiol 30:207–229
Mark H, Workman J (eds) (1991) Statistics in spectroscopy. Academic, New York
Westerhaus MO (1989) In: Marten GC, Shenk JS, Barton FE (eds) Near infrared reflectance spectroscopy (NIRS): analysis of forage quality, US Dep Agric Handb 643:38–39
Williams PC, Sobering DC (1993) J Near Infrared Spectrosc 1:25–32
Murray I, Williams P (1987) In: Williams P, Norris K (eds) Near-infrared technology in the agricultural and food industries. American Association of Cereal Chemists, Minnesota, pp 17–34
Ruano Ramos A, García Ciudad A, García Criado B (1999) J Sci Food Agric 79:137–143
Redshaw ES, Mathison GW, Milligan LP, Weisenburger RD (1986) Can J Anim Sci 66:103–115
Valdes EV, Young LG, McMillan I, Winch JE (1985) Can J Anim Sci 65:753–760
Shenk JS, Landa I, Hoover MR, Westerhaus MO (1981) Crop Sci 21:355–358
Convertini G, Ferri D, Lanza F, Cilardi AM (1991) In: Biston R, Bartiaux-Thill N (eds) Proceeding of 3rd international conference near infrared spectroscopy 2, Agricultural Research Center, Gembloux, pp 522–540
Naes T, Martens H (1985) Commun Stat Simul Comput 14:545–576
Otto M, Wegscheider W (1985) Anal Chem 57:63–69
Lindberg W, Persson JA, Wold S (1983) Anal Chem 55:643–648
Shenk JS, Westerhaus MO (1991) Crop Sci 31:1548–1555
Shenk JS, Westerhaus MO (1993) Monograph: analysis of agriculture and food products by near-infrared reflectance spectroscopy. Infrasoft, Port Matilda
Osborne BG, Fearn T (eds) (1986) Near infrared spectroscopy in food analysis. Longman Sci Technol, Harlow, p 200
Acknowledgements
This work was supported by projects JCyL (CSI 6/01), and MCyT (AGL2002–02766 AGR-FOR). Thanks are also due J.C. Estévez, M. Miguélez and Y. Arnaiz for technical assistance. We are grateful to two anonymous reviewers for valuable comments on an earlier draft of the manuscript.
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Petisco, C., García-Criado, B., Vázquez de Aldana, B.R. et al. Use of near-infrared reflectance spectroscopy in predicting nitrogen, phosphorus and calcium contents in heterogeneous woody plant species. Anal Bioanal Chem 382, 458–465 (2005). https://doi.org/10.1007/s00216-004-3046-7
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DOI: https://doi.org/10.1007/s00216-004-3046-7