Abstract
A rapid high-performance liquid chromatography (HPLC) method using a monolithic column with UV detection at 238 nm was developed for the determination of fenpropathrin, betacyfluthrin, deltamethrin, and permethrin (cis and trans isomers) in whole urine. The method is based on the use of a monolithic chromatographic column and a restricted access material (RAM) cartridge for sample preparation. The mobile phase was water/acetonitrile (42:58 v/v), the flow rate was 3 mL min−1, and chromatographic separation was carried out in 10 min. The separation of cis and trans isomers of permethrin was also possible under the above-mentioned conditions. Detection limits in reconstituted whole urine samples were between 0.9 μg L−1 for betacyfluthrin and 4.4 μg L−1 for fenpropathrin and trans-permethrin. Recoveries for urine samples spiked with different amounts of pyrethroids (between 19 μg L−1 and 75 μg L−1) were in the 70±6 to 90±7% range.
Similar content being viewed by others
References
Smith PA, Thompson MJ, Edwards JW (2002) J Chromatogr B 778:113–120
Barr DB, Needham LL (2002) J Chromatogr B 778:5–29
Di Muccio A, Pelosi P, Barbini DA, Generali T, Girolimetti S, Stefanelli P, Leonelli A, Amendola G,Vergori L, Viana Fresquet E (1999) J Chromatogr A 833:19–34
Lentza-Rizos Ch, Avramides EJ, Visi E (2001) J Chromatogr A 921:297–304
Sanusi A, Guillet V, Montury M (2004) J Chromatogr A 1046:35–40
Elflein L, Berger-Preiss E, Preiss A, Elend M, Levsen K, Wünsch G (2003) J Chromatogr B 795:195–207
Ramesh A, Ravi PE (2004) J Chromatogr B 802:271–376
López-López T, Gil-García M.D, Martínez-Vidal JL, Martínez-Galera M (2001) Anal Chim Acta 447:101–111
Tanaka N, Kobayashi H, Ishizuka N, Minukuchi H, Nakanishi K, Hosoya K, Ikegami T (2002) J Chromatogr A 965:35–49
Hogendoor EA, Huls R, Dijkman E, Hoogerbrugge R (2001) J Chromatogr A 938:23–33
Acknowledgments
The authors wish to thank the Spanish Ministerio de Ciencia y Tecnología (Dirección General de Investigación) for financial support (project BQU-2003–00667).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
León-González, M., Plaza-Arroyo, M., Pérez-Arribas, L. et al. Rapid analysis of pyrethroids in whole urine by high-performance liquid chromatography using a monolithic column and off-line preconcentration in a restricted access material cartridge. Anal Bioanal Chem 382, 527–531 (2005). https://doi.org/10.1007/s00216-004-3042-y
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00216-004-3042-y