Abstract
A procedure has been developed for determining the selenium in cow’s milk using hydride generation–atomic absorption spectrometry (HG-AAS) following microwave-assisted acid digestion. The selenium distributions in milk whey, fat and micellar casein phases were studied after separating the different phases by ultracentrifugation and determining the selenium in all of them. The detection limits obtained by HG-AAS for the whole milk, milk whey and micellar casein were 0.074, 0.065 and 0.075 μg l−1, respectively. The accuracy for the whole milk was checked by using a Certified Reference Material CRM 8435 whole milk powder from NIST, and the analytical recoveries for the milk whey and casein micelles were 100.9 and 96.9%, respectively. A mass balance study of the determination of selenium in the different milk phases was carried out, obtaining values of 95.5–100.8%. The total content of selenium was determined in 37 milk samples from 15 different manufacturers, 19 whole milk samples and 18 skimmed milk samples. The selenium levels found were within the 8.5–21 μg l−1 range. The selenium distributions in the different milk phases were studied in 14 whole milk samples, and the highest selenium levels were found in milk whey (47.2–73.6%), while the lowest level was found for the fat phase (4.8–16.2%). A strong correlation was found between the selenium levels in whole milk and the selenium levels in the milk components.
Similar content being viewed by others
References
Köhrle J, Brigelius-Flohe R, Bock A, Gartner R, Meyer O, Floe L (2000) Biol Chem 381:849–864
Ursini F, Heim S, Kiess M, Maiorino M, Wissing J, Flohe L (1999) Science 285:1393–1396
Martin-Romero F, Kryukov G, Lobanov A, Carlson B, Lee B, Gladyshev V, Hatfield L (2001) J Biol Chem 276:29798–29804
Schmutzler C, Köhrle J (2000) Eur J Endocrinol 193:15–24
Rayman MP (2000) Lancet 356:233–241
Clark LC, Coms GF, Turnbull BW, Slate EH, Chalker DK, Chow J, Davis LS, Glover RA, Graham GF, Gross EG, Krongrad A, Lesher JL, Park HK, Sanders BB, Smith CL, Taylor JR (1996) J Am Med Assoc 276:1957–1963
Spallholz JE, Palace VP, Reid TW (2004) Biochem Pharmacol 67:547–554
Corcoran NM, Najdovska M, Costello AJ (2004) J Urology 171:907–910
Whanger PD (2004) Brit J Nutr 91:11–28
Fridovich I (1974) Adv Enzymol RAMB 41:35–97
Maddipati KR, Marnett LJ (1987) J Biol Chem 262:17398–17403
Monsen ER (2000) J Am Diet Assoc 100:637–640
Schrauzer GN, White DA (1978) Bioinorg Chem 8:303–318
Combs G (2001) Nutr Cancer 40:6–11
Oster O, Prellwitz (1989) Biol Trace Elem Res 20:1–14
Matek M, Blanusa M, Grgic J (2000) Eur Food Res Technol 210:155–160
Maihara VA, Gonzaga IB, Silva VL, Favaro DIT, Vasconcellos MBA, Cozzolino SMF (2004) J Radioanal Nucl Chem 259:465–468
Yang FY, Lin ZH, Li SG, Guo BO, Yin YS (1998) J Trace Elem Elect H 2:157–163
Aspila P (1991) J Agr Sci Finland 63:1–74
Inam R, Somer G (2000) Food Chem 69:345–350
Martino FAR, Sanchez MLF, Sanz-Medel A (2001) Anal Chim Acta 442:191–200
Klapeck T, Mandić ML, Grgić J, Primorac Lj, Perl A, Krstanović V (2004) Food Chem 85:445–452
Murphy J, Cashman KD (2001) Food Chem 74:493–498
Bermejo P, Barciela J, Peña EM, Bermejo A, Fraga JM, Cocho JA (2001) J Anal Atom Spectrom 16:188–193
Rodríguez-Rodríguez EM, Sanz-Alejos M, Diaz-Romero C (1999) J Agr Food Chem 47:1520–1524
Rodríguez-Rodríguez EM, Sanz-Alejos M, Diaz-Romero C (2001) J Food Comp Anal 14:419–430
Lindmark-Månsson H, Fondén R, Pettersson HE (2003) Int Dairy J 13:409–425
Al-Awadi FM, Srikumar TS (2001) J Trace Elem Exp Med 14:57–67
Aleixo PC, Nóbrega JA (2003) Food Chem 83:457–462
Licata P, Trombetta D, Cristani M, Giofré F, Martino D, Calo M, Naccari F (2004) Environ Int 30:1–6
Cava-Montesinos P, Cervera ML, Pastor A, De la Guardia M (2003) Anal Chim Acta 481:291–300
Cava-Montesinos P, Cervera ML, Pastor A, De la Guardia M (2004) Talanta 62:175–184
Cava-Montesinos P, Cervera ML, Pastor A, De la Guardia M (2004) J Anal Atom Spectrom 19:696–699
Givens DJ, Allinson R, Cotrill B, Blake JS (2004) J Sci Food Agric 84:811–817
Shen L, Van Dael P, Deelstra H (1993) Z Lebensm Unters For 197:342–345
Bermejo P, Domínguez R, Bermejo A (1997) Talanta 45:325–330
Bermejo P, Domínguez R, Bermejo A (1997) Fresen J Anal Chem 357:457–461
Manugistics (1992) Reference manual for Statgraphics Plus version 5.0. Manugistics, Inc., Rockville, MD
Acknowledgements
The authors gratefully acknowledge the support of this work by FEIRACO Sociedad Cooperativa Gallega.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Muñiz-Naveiro, Ó., Domínguez-González, R., Bermejo-Barrera, A. et al. Determination of total selenium and selenium distribution in the milk phases in commercial cow’s milk by HG-AAS. Anal Bioanal Chem 381, 1145–1151 (2005). https://doi.org/10.1007/s00216-004-3010-6
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00216-004-3010-6