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Trace analysis of rapamycin in human blood by micellar electrokinetic chromatography

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Abstract

A capillary electrophoretic method with UV detection at 278 nm has been developed for analysis of the immunosuppressant rapamycin (sirolimus) in human blood at low microgram per liter levels. Separation has been achieved in an acidic carrier electrolyte containing sodium dodecylsulfate and 30% (v/v) acetonitrile. For sample clean-up and preconcentration, an off-line solid-phase extraction step using a silica-based reversed-phase material and an on-capillary focussing technique were employed. The latter allows the injection of increased sample volumes without excessive band broadening. Although this new method is less sensitive than existing liquid chromatographic procedures combined with mass spectrometry, it is fully suited to routine analysis of rapamycin in blood from patients treated with this drug. Last but not least the low costs make it an attractive alternative to established methods.

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Authors and Affiliations

  1. Institute for Analytical Chemistry, Johannes-Kepler-University Linz, Altenbergerstraße 69, 4040, Linz, Austria

    Wolfgang Buchberger, Matthias Ferdig & Thuy Diep Thanh Vo

  2. Institute of Medical and Chemical Laboratory Diagnostics, Wagner-Jauregg-Hospital, Wagner-Jauregg-Weg 15, 4020, Linz, Austria

    Rudolf Sommer

Authors
  1. Wolfgang Buchberger
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  2. Matthias Ferdig
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  3. Rudolf Sommer
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  4. Thuy Diep Thanh Vo
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Correspondence to Matthias Ferdig.

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Buchberger, W., Ferdig, M., Sommer, R. et al. Trace analysis of rapamycin in human blood by micellar electrokinetic chromatography. Anal Bioanal Chem 380, 68–71 (2004). https://doi.org/10.1007/s00216-004-2687-x

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  • DOI: https://doi.org/10.1007/s00216-004-2687-x

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