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Poly(dimethylsiloxane) microchip capillary electrophoresis with electrochemical detection for rapid measurement of acetaminophen and its hydrolysate

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Abstract

Poly(dimethylsiloxane) microchip capillary electrophoresis with amperometric detection has been used for rapid separation and determination of acetaminophen and its hydrolysate, i.e. p-aminophenol. A Pt ultramicroelectrode with a diameter of 10 μm positioned at the outlet of the separation channel was used as a working electrode for amperometric detection. Factors influencing separation and detection were investigated and optimized. Results show that acetaminophen and p-aminophenol can be well separated within 35 s with RSD<1% for migration time and <7% for detection current for both analytes. Detection limits for both analytes are estimated to be 5.0 μmol L−1 (approximately 0.1 fmol) at S/N=3. This method has been successfully applied to the detection of traces of p-aminophenol in paracetamol tablets.

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Acknowledgements

This work was supported by the Grants from the National Natural Science Foundation of China (Grant No.20299033) and the Ministry of Education of China (No. 20020284021).

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Authors and Affiliations

  1. The State Key Laboratory of Coordination Chemistry, Institute of Analytical Science, Department of Chemistry, Nanjing University, Nanjing, 210093, China

    F. Y. He, A. L. Liu & X. H. Xia

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  1. F. Y. He
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  2. A. L. Liu
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  3. X. H. Xia
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Corresponding author

Correspondence to X. H. Xia.

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He, F.Y., Liu, A.L. & Xia, X.H. Poly(dimethylsiloxane) microchip capillary electrophoresis with electrochemical detection for rapid measurement of acetaminophen and its hydrolysate. Anal Bioanal Chem 379, 1062–1067 (2004). https://doi.org/10.1007/s00216-004-2680-4

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  • DOI: https://doi.org/10.1007/s00216-004-2680-4

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