Analytical and Bioanalytical Chemistry

, Volume 379, Issue 5–6, pp 861–866 | Cite as

Speciation of selenium in selenium-enriched shiitake mushroom, Lentinula edodes

  • Yasumitsu OgraEmail author
  • Kazuya Ishiwata
  • Jorge Ruiz Encinar
  • Ryszard Łobiński
  • Kazuo T. Suzuki
Original Paper


The major selenium compound in an aqueous extract of the most popular mushroom in Eastern Asian countries, shiitake (Lentinula edodes), fortified with selenium (Se) was identified by means of hyphenated techniques, i.e. HPLC-inductively coupled argon plasma mass spectrometry and HPLC-electrospray ionization mass spectrometry (HPLC–ICP MS and HPLC–ESI MS). Sixty-eight per cent of the total Se in the selenized shiitake was extracted with water, and 49.8% of the Se in the water extract was eluted in the high molecular mass fraction (>40,000 kDa) before incubation at 37 °C. After incubation, 40.6% of the Se in the water extract was eluted in a lower molecular mass fraction and the Se eluted in the high molecular mass fraction had decreased to 14.0%, suggesting that the major selenium compound in the water extract was initially in a form bound to macromolecule(s) and was then enzymatically liberated from the macromolecule(s). The retention time of the liberated selenium compound in HPLC–ICP MS matched that of selenomethionine (SeMet), and the masses of molecular and fragment ions detected by HPLC–ESI MS also suggested that the selenium compound was SeMet. The selenized shiitake accumulated Se as SeMet, and SeMet might be bound to the water extractable high molecular mass protein(s).


Selenium Speciation Lentinula edodes ICP MS ESI MS Selenomethionine 



Financial support from the Japan–Europe (France) Research Cooperative Program of the Japan Society for the Promotion of Science is gratefully acknowledged. KTS and YO also wish to acknowledge the Grant-in-Aid from the Ministry of Education, Culture, Science, Sports and Technology of Japan (Grant No. 14657587).

Supplementary material


216_2004_2670.pdf (101 kb)
(pdf 101 KB)


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Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • Yasumitsu Ogra
    • 1
    Email author
  • Kazuya Ishiwata
    • 1
  • Jorge Ruiz Encinar
    • 2
  • Ryszard Łobiński
    • 2
    • 3
  • Kazuo T. Suzuki
    • 1
  1. 1.Graduate School of Pharmaceutical SciencesChiba UniversityChuoJapan
  2. 2.CNRS UMR 5034PauFrance
  3. 3.Department of ChemistryWarsaw University of TechnologyWarsawPoland

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