Abstract
Reassembly of protein from its peptide fragments is a technique that can have many applications in the bioanalytical field. Typically, a reporter protein fragmented into its two peptides is employed as a label in this study. This fragments of peptide can reassemble yielding an active functional reporter. This reassembly of the protein can be assisted by non-covalently interacting peptides or proteins, which are attached to the fragmented reporter. This technique has been employed in several applications including study of protein–protein interactions, antibody screening, immunoassays, and high-throughput screening. This review focuses on different reporters employed in the study of reassembly of proteins and applications of this strategy in bioanalysis.
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Acknowledgements
The author would like to thank Prof. Sylvia Daunert from the University of Kentucky for her guidance. This work was supported by grant number P42 ES 07380 from the National Institute of Environmental Health Sciences, National Institutes of Health, and National Science Foundation.
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Deo, S.K. Exploring bioanalytical applications of assisted protein reassembly. Anal Bioanal Chem 379, 383–390 (2004). https://doi.org/10.1007/s00216-004-2633-y
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DOI: https://doi.org/10.1007/s00216-004-2633-y