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Investigation of metal-binding metallothioneins in the tissues of rats after oral intake of cinnabar

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Abstract

The multi-metal-binding MT fractions in rat tissues after oral intake of cinnabar were characterized by hyphenated size-exclusion chromatography (SEC) and inductively coupled plasma mass spectrometry (ICP–MS). With the increase of both the feeding time and dosage of cinnabar the amounts of Hg-binding MT fractions in rat kidney of groups fed cinnabar increased significantly compared with the control group. Meanwhile, more Cu-binding MT were synthesized in the rat kidney, which confirmed the manipulating effect of MT in the homeostasis of Cu for detoxification of nephritic mercury. Although the Hg-binding MT fractions in rat liver of all cinnabar groups were almost independent of cinnabar dosage (2.5–5.0 g kg−1 bw) and feeding time (2–4 weeks), the amount was much higher than that of the control group. The amounts of Hg-MT in other rat tissues of the cinnabar groups and the control group were compared and their significance is discussed.

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Acknowledgements

Financially supported by the Key Project of the National Natural Scientific Foundational of China (20175022). The animal treatment was accomplished at the Institute of Materia Medica of Chinese Academy of Medical Sciences and Peking Union Medical College.

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Authors and Affiliations

  1. College of Bioengineering, Jimei University, 361021, Xiamen, P.R. China

    Zhi-Yong Huang

  2. QingDao Key Lab of Analytical Technology Development and Standardization of Chinese Medicines, First Institute Oceanography, S.O.A, 266061, Qingdao, P.R. China

    Zhi-Xia Zhuang, Xiao-Ru Wang & Frank S. C. Lee

  3. The Key Laboratory of Analytical Science of Ministry of Education of China, Xiamen University, 361005, Xiamen, P.R. China

    Jin-Can Shen & Zhi-Xia Zhuang

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  1. Zhi-Yong Huang
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  2. Jin-Can Shen
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  3. Zhi-Xia Zhuang
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  4. Xiao-Ru Wang
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  5. Frank S. C. Lee
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Correspondence to Xiao-Ru Wang.

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Huang, ZY., Shen, JC., Zhuang, ZX. et al. Investigation of metal-binding metallothioneins in the tissues of rats after oral intake of cinnabar. Anal Bioanal Chem 379, 427–432 (2004). https://doi.org/10.1007/s00216-004-2624-z

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  • DOI: https://doi.org/10.1007/s00216-004-2624-z

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