Skip to main content
SpringerLink
Log in

Hyphenated techniques for speciation of Se in in vitro gastrointestinal digests of Saccharomyces cerevisiae

  • Original Paper
  • Published:
Analytical and Bioanalytical Chemistry Aims and scope Submit manuscript

Abstract

A method was developed allowing the separation, detection and identification of Se species extracted from yeast supplements during simulated digestion processes. The in vitro gastric and intestinal digests were studied for their Se compounds by successive high-performance liquid chromatography–inductively coupled plasma mass spectrometry (HPLC-ICP-MS) and high-performance liquid chromatography–electrospray tandem mass spectrometry (HPLC-ES-MS-MS) analyses. The conditions for the separation were chosen as to be compatible with both ICP-MS and ES-MS-MS detection. HPLC-ICP-MS was used to screen the extracts for their Se content. By means of HPLC-ES-MS-MS, the compounds extracted were identified on-line according to their retention time, m/z of the molecular ion and the presence of typical product ions. From these results, it was clear that the main compound extracted by both gastric and intestinal fluid was Se-methionine, which was also the main Se compound extracted by proteolytic digestion from the yeast supplements. Two other minor compounds could be identified as Se-cystine and Se(O)-methionine, a degradation product of Se-methionine.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7

Similar content being viewed by others

References

  1. Schrauzer G (2000) Recent Adv Nutr Sci 1653–1656

  2. Dumont E, De Cremer K, Van Hulle M, Chéry CC, Vanhaecke F, Cornelis R (2004) J Anal At Spectrom 19:167–171

    Article  CAS  Google Scholar 

  3. Dumont E, De Cremer K, Van Hulle M, Chéry CC, Vanhaecke F, Cornelis R (2004) Analyst (submitted)

  4. Wrobel K, Wrobel K, Kannamkumarath S, Caruso J (2003) Anal Bioanal Chem 377:670–674

    Article  CAS  PubMed  Google Scholar 

  5. Cao T, Cooney R, Woznichak M, Sheldon M, Browner M (2001) Anal Chem 73:2898–2902

    Google Scholar 

  6. Gammelgaard B, Grimstrup Madsen K, Bjerrum J, Bendahl L, Jøns O, Olsen J, Sidenius U (2003) J Anal At Spectrom 18:65–70

    Article  CAS  Google Scholar 

  7. Ogra Y, Ishiwata K, Takayama H, Aimi N, Suzuki K (2002) J Chromatogr B 767:301

    CAS  Google Scholar 

  8. Morisi G, Menditto A, Chiodo F, Minoprio A, Mosca M, Patriarca M (1998) Fresenius J Anal Chem 360:489–493

    Article  CAS  Google Scholar 

  9. Labat L, Dehon B, Lhermitte M (2003) Anal Bioanal Chem 376:270–273

    CAS  PubMed  Google Scholar 

  10. Crews H, Clarke P, Lewis J, Owen L, Strutt P, Izquierdo A (1996) J Anal At Spectrom 11:1177–1182

    CAS  Google Scholar 

  11. Fairweather-Tait S, Dainty J (2002) Food Addit Contam 19:939–947

    Google Scholar 

  12. Fairweather-Tait S (1999) Fresenius J Anal Chem 363:536–540

    Article  CAS  Google Scholar 

  13. Basu T, Donaldson D (2003) Best Pract Res Cl Ga 17:957–979

    Article  CAS  Google Scholar 

  14. US Pharmacopeia XXIV & national formulary 19 (2000) The United States pharmacopeial convention, Rockville, pp 2235–2236

  15. Mc Sheehy S, Mester Z (2003) Trends Anal Chem 22:210–224

    Article  Google Scholar 

  16. Larsen E, Hansen M, Fan T, Vahl M (2001) J Anal At Spectrom 16:1403–1408

    CAS  Google Scholar 

Download references

Acknowledgements

E.D. is a grant holder of the Institute for the Promotion of Innovation by Science and Technology in Flanders (IWT). The authors want to thank L. Mees for doing all INAA experiments.

Author information

Authors and Affiliations

  1. Department of Analytical Chemistry, Ghent University, Proeftuinstraat 86, 9000, Gent, Belgium

    Emmie Dumont, Frank Vanhaecke & Rita Cornelis

Authors
  1. Emmie Dumont
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Frank Vanhaecke
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Rita Cornelis
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Emmie Dumont.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Dumont, E., Vanhaecke, F. & Cornelis, R. Hyphenated techniques for speciation of Se in in vitro gastrointestinal digests of Saccharomyces cerevisiae . Anal Bioanal Chem 379, 504–511 (2004). https://doi.org/10.1007/s00216-004-2615-0

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00216-004-2615-0

Keywords

Search

Navigation