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Determination of ginkgolide A, B, and bilobalide in biloba L. extracts by microdialysis-HPLC

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Abstract

A method has been developed for measuring ginkgolide A, B, and bilobalide in ginkgo biloba L. extracts by using a self-assembled microdialysis device coupled on-line to high-performance liquid chromatography. The optimal conditions for dialysis efficiency and chromatographic analysis were investigated. The results show that the dialysis efficiencies for ginkgolide A, B, and bilobalide are 97.8–100.4%. For the spike concentration of 45 mg L−1, the recoveries of ginkgolide A, B, and bilobalide in ginkgo biloba L. extracts were at 97–100.7% with 3.1–4.7% RSD. The proposed method has therefore been shown to be applicable to the analysis of ginkgolide A, B, and bilobalide in ginkgo biloba L. extracts.

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Authors and Affiliations

  1. Department of Chemistry, National Kaohsiung Normal University, 116 Ho-Ping 1st Rd., Kaohsiung, Taiwan

    Houn-Lin Chiu, Hui-Ya Lin & Thomas Ching-Cherng Yang

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  1. Houn-Lin Chiu
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  2. Hui-Ya Lin
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  3. Thomas Ching-Cherng Yang
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Correspondence to Thomas Ching-Cherng Yang.

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Chiu, HL., Lin, HY. & Yang, T.CC. Determination of ginkgolide A, B, and bilobalide in biloba L. extracts by microdialysis-HPLC. Anal Bioanal Chem 379, 445–448 (2004). https://doi.org/10.1007/s00216-004-2606-1

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  • DOI: https://doi.org/10.1007/s00216-004-2606-1

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