Abstract
A super-modified simplex (SMS) method has been used to optimize the mobile phase used for separation of seven water-soluble vitamins in multivitamin tablets by gradient micellar liquid chromatography (MLC) with ultraviolet (UV) detection at 254, 295, and 361 nm. Effect of column temperature and addition of organic modifier to the mobile phase on separation efficiency were investigated: the appropriate conditions used were a temperature of 35°C and 1-butanol modifier. The sodium dodecyl sulfate (SDS) concentration, pH, and 1-butanol% in the mobile phase were chosen for simultaneous optimization using the SMS method. The optimum mobile phase was found to be 16 mmol L−1 (mM) SDS, 0.02 M phosphate buffer, pH 3.6, and a gradient of 3.5–10% (v/v) butanol. The total analysis time for vitamins was 75 min. The analytical parameters including linearity (r>0.9970), limit of detection (0.12–50 µg mL−1), precision of method (relative standard deviation (RSD) <8.90%), and accuracy obtained by the recovery assay (88–103%) support the usefulness of the proposed method for the determination of the water-soluble vitamins.
Similar content being viewed by others
References
Moreno P, Salvado V (2000) J Chromatogr A 870:207–215
Cho CM, Ko JH, Cheng WJ (2000) Talanta 51:799–806
Albala-Hurado S, Veciana-Nogues MT, Izquierdo-Pulido M, Marine-font A (1997) J Chromatogr A 778:247–253
Iwase H (1992) J Chromatogr 625: 377–381
Blanco D, Sanchez LA, Gutierrez MD (1994) J Liq Chrom 17:1525–1539
Monferrer-Pons L, Capella-peiro ME, Gil-Agusti M, Esteve-Romero J (2003) J Chromatogr A 984:223–231
Hernandez MJM, Alvarez-Coque MCG (1992) Analyst 117:831–837
Morgan SL, Deming SN (1975) J Chromatogr 112:267–285
Deming SN, Morgan SL (1973) Anal Chem 45:278A–283A
Nelder JA, Mead R (1965) Comp J 7:308–313
Routh MW, Swartz PA, Denton MB (1977) Anal Chem 49:1422–1428
Berridge JC, Morrissey EG (1984) J Chromatogr 316:69–79
Madamba-Tan LS, Strasters JK, Khaledi MG (1994) J Chromatogr A 683:335–345
Madamba-Tan LS, Strasters JK, Khaledi MG (1994) J Chromatogr A 683:321–334
Miller JC, Miller JN (eds) (1992) Statistics for analytical chemistry. Ellis Horwood, Chichester
Acknowledgements
The authors gratefully acknowledge support of this work by the University of Isfahan Graduate Office and Research Council and Amin Pharmaceutical Company.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Ghorbani, A.R., Momenbeik, F., Khorasani, J.H. et al. Simultaneous micellar liquid chromatographic analysis of seven water-soluble vitamins: optimization using super-modified simplex. Anal Bioanal Chem 379, 439–444 (2004). https://doi.org/10.1007/s00216-004-2594-1
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00216-004-2594-1