Abstract
Microstructures constructed from SU-8 polymer and produced on CaF2 base plates have been developed for microchip-based analysis systems used to perform FTIR spectroscopic detection using mid-IR synchrotron radiation. The high brilliance of the synchrotron source enables measurements at spot sizes at the diffraction limit of mid-IR radiation. This corresponds to a spatial resolution of a few micrometers (5–20 μm). These small measurement spots are useful for lab-on-a-chip devices, since their sizes are comparable to those of the structures usually used in these devices. Two different types of microchips are introduced here. The first chip was designed for time-resolved FTIR investigations of chemical reactions in solution. The second chip was designed for chip-based electrophoresis with IR detection on-chip. The results obtained prove the operational functionality of these chips, and indicate the potential of these new devices for further applications in (bio)analytical chemistry.
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Acknowledgements
Acknowledgement is given to the Austrian Science Fund for support within the project FWF 15531. Furthermore, financial support from the ANKA for carrying out the measurements in Karlsruhe is gratefully acknowledged. J.R.B. also acknowledges support from the Spanish Ministry of Culture, Sport and Science.
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Kulka, S., Kaun, N., Baena, J.R. et al. Mid-IR synchrotron radiation for molecular specific detection in microchip-based analysis systems. Anal Bioanal Chem 378, 1735–1740 (2004). https://doi.org/10.1007/s00216-004-2534-0
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DOI: https://doi.org/10.1007/s00216-004-2534-0