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Optimization of solid-phase extraction procedure for determination of polychlorinated dibenzo-p-dioxins, polychlorinated dibenzofurans, and coplanar polychlorinated biphenyls in humic acid containing water

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Abstract

A solid-phase extraction (SPE) method was optimized for accurate determination of polychlorinated dibenzo-p-dioxins (PCDDs), polychlorinated dibenzofurans (PCDFs), and coplanar polychlorinated biphenyls (CoPCBs) in humic acid containing surface water. Recovery experiments using humic materials revealed that humic acids permit dioxins to pass through an octadecylsilica (C18) extraction disk by associating with them under weakly alkaline conditions. Acidification of the sample before percolation improved this otherwise insufficient recovery. The analysis of surface water acidified to pH 2 gave better recovery with surrogate standards and lower quantitative values for higher-chlorinated homologues than the sample at pH 9. In all samples, the native octachlorodibenzo-p-dioxin (OCDD) peak abundance showed no difference between at pH 2 and at pH 9, indicating overestimation of the quantitative value of the homologue at pH 9. Acidification of a humic acid containing water sample can avoid overestimation of higher-chlorinated congeners caused by insufficient recovery of their corresponding surrogates.

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References

  1. Fiedler H, Van den Berg M (1996) Environ Sci Pollut Res 3:122–128

    Google Scholar 

  2. Tyskling M, Lundgren K, Eriksson L, Sjostorm M, Rappe C (1993) Chemosphere 27:47–54

    Article  Google Scholar 

  3. Japanese Industrial Standards Committee (1999) Japanese industrial standard (JIS K 0312)

  4. US EPA Office of Water Regulations and Standards (1990) Method 1613 (revision A)

  5. Thurman EM (1985) Organic geochemistry of natural waters. Martinus Nijhoff/Dr W  Junk Publishers, Dordrecht

  6. Servos MR, Muir DCG, Webster GRB (1989) Aquat Toxicol 14:169–184

    Article  CAS  Google Scholar 

  7. McCarthy JF, Jimenez BD (19854–1941) Environ Sci Technol 19:1072–1076

  8. Schlautman MA, Morgan JJ (1993) Environ Sci Technol 27:961–969

    CAS  Google Scholar 

  9. Li N, Lee HK (2000) Anal Chem 72:5272–5279

    Article  CAS  PubMed  Google Scholar 

  10. Ramos EU, Wouter HJ Vaes, Henk JM Verhaar, Joop LM Hermens (1997) Environ Toxicol Chem 16:2229–2231

    CAS  Google Scholar 

  11. Chiou CT, Kile DE, Brinton TI, Malcolm RL, Leenheer JA (1987) Environ Sci Technol 21:1231-1234

    CAS  Google Scholar 

  12. Johnson WE, Fendinger NJ, Plimmer JR (1991) Anal Chem 63:1510–1513

    CAS  PubMed  Google Scholar 

  13. Focant JF, Eppe G, Pirard C, Pauw ED (2001) J Chromatogr A 925:207–221

    Article  CAS  PubMed  Google Scholar 

  14. Taylor KZ, Waddell DS, Reiner EJ, MacPherson KA (1995) Anal Chem 67:1186–1190

    CAS  Google Scholar 

  15. Pichon V, Cau Dit Coumes C, Chen L, Guenu S, Hennion MC (1996) J Chromatogr A 737:25–33

    Article  CAS  Google Scholar 

  16. Malcolm RL, MacCarthy P (1986) Environ Sci Technol 20:904–911

    CAS  Google Scholar 

Download references

Acknowledgements

We thank Dr M Shinomiya (National Environmental Research and Training Institute, Ministry of the Environment) for help in measuring the DOC concentration in the water samples.

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Authors and Affiliations

  1. National Environmental Research and Training Institute, Ministry of the Environment, 3–3 Namiki, Tokorozawa-shi, 359–0042, Saitama, Japan

    Hiroaki Otaka

  2. Japan Quality Assurance Organization, 14–12 Dezuminato, Chuo-ku, Chiba-shi, 260–0023, Chiba, Japan

    Hisao Shimono

  3. National Institute for Environmental Studies, 16–9 Onogawa, Tsukuba-shi, 305–8506, Ibaraki, Japan

    Shunji Hashimoto

Authors
  1. Hiroaki Otaka
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  2. Hisao Shimono
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  3. Shunji Hashimoto
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Correspondence to Hiroaki Otaka.

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Otaka, H., Shimono, H. & Hashimoto, S. Optimization of solid-phase extraction procedure for determination of polychlorinated dibenzo-p-dioxins, polychlorinated dibenzofurans, and coplanar polychlorinated biphenyls in humic acid containing water. Anal Bioanal Chem 378, 1854–1860 (2004). https://doi.org/10.1007/s00216-004-2510-8

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  • DOI: https://doi.org/10.1007/s00216-004-2510-8

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