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Analytical and Bioanalytical Chemistry

, Volume 377, Issue 7–8, pp 1120–1123 | Cite as

Feasibility study: fast liquid chromatography–mass spectrometry for the quantification of aspartic acid in an aspartate drug

  • Jochen SchmidtEmail author
Special Issue Paper

Abstract

We have studied the feasibility of fast high-performance liquid chromatography coupled to electrospray ionization mass spectrometry in the selected ion monitoring mode for the quantitative determination of aspartic acid in an aspartate drug. Internal standardization was required, but mass spectrometric detection allowed for very short retention times of approximately 0.5 min for the analyte and the internal standard without chromatographic separation. The analytical system was found stable, as demonstrated by multiple injections giving a coefficient of variation of 4% for the peak area ratio of aspartic acid and glutamic acid. Calibrations were linear between 0.5 ng and 150 ng aspartic acid injected, with accuracies between 99.8% and 102% found for the back-calculated amounts. Investigation of several drug batches gave reasonable results. Therefore, the method appeared feasible for the determination of aspartic acid in an aspartate drug from 0.3 wt% to 100 wt% aspartic acid.

Keywords

Quantification Aspartic acid Aspartate drug Fast HPLC-MS Electrospray ionization Selected ion monitoring 

Abbreviations

CV

coefficient of variation

ESI

electrospray ionization

HPLC

high-performance liquid chromatography

HSGC

headspace gas chromatography

KaFi

Karl Fischer titration

MS

mass spectrometry

MSD

mass-selective detector

% m/m

percentage by weight

PAR

peak area ratio

r2

coefficient of determination

RP

reversed-phase

SIM

selected ion monitoring

Notes

Acknowledgements

The skilled technical assistance of Katrin Grießhammer and Adrian Hartleib is greatfully acknowledged.

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Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  1. 1.Preclinical Bioanalytical ChemistrySolvay Pharmaceuticals, Discovery HannoverHannoverGermany

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