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Cholinesterase sensors based on screen-printed electrodes for detection of organophosphorus and carbamic pesticides

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Abstract

Cholinesterase sensors based on screen-printed electrodes modified with polyaniline, 7,7′,8,8′-tetracyanoquinodimethane (TCNQ), and Prussian blue have been developed and tested for detection of anticholinesterase pesticides in aqueous solution and in spiked grape juice. The influence of enzyme source and detection mode on biosensor performance was explored. It was shown that modification of the electrodes results in significant improvement of their analytical characteristics for pesticide determination. Thus, the slopes of the calibration curves obtained with modified electrodes were increased twofold and the detection limits of the pesticides were reduced by factors of 1.6 to 1.8 in comparison with the use of unmodified transducers. The biosensors developed make it possible to detect down to 2×10−8 mol L−1 chloropyrifos-methyl, 5×10−8 mol L−1 coumaphos, and 8×10−9 mol L−1 carbofuran in aqueous solution and grape juice. The optimal conditions for grape juice pretreatment were determined to diminish interference from the sample matrix.

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Abbreviations

ChE:

Cholinesterase

TCNQ:

7,7′,8,8′-Tetracyanoquinodimethane

ChO:

Choline oxidase

AChE:

Acetylcholinesterase

BChE:

Butyrylcholinesterase

BSA:

Bovine serum albumin

2-PAM:

2-Pyridine aldoxime methiodide

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Acknowledgements

Financial support of INTAS (grant No 00-273) is gratefully acknowledged. The authors also thank Professor A. A. Karyakin, Moscow State University, who provided the techniques for polyaniline doping and electrode modification and Professor G. P. Karpacheva, Institute for Petrochemical Synthesis, Russian Academy of Sciences, Moscow, for the synthesized polyaniline.

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Authors and Affiliations

  1. Chemistry Department, Kazan State University, 18 Kremlevskaya Street, 420008, Kazan, Russia

    Alexey Ivanov, Gennady Evtugyn & Herman Budnikov

  2. Dipartimento di Science e Technologie Chimiche, Universita di Roma "Tor Vergata" via della Ricerca Scientifica, 00133, Roma, Italy

    Francesco Ricci, Danila Moscone & Giuseppe Palleschi

Authors
  1. Alexey Ivanov
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  2. Gennady Evtugyn
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  3. Herman Budnikov
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  4. Francesco Ricci
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  5. Danila Moscone
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  6. Giuseppe Palleschi
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Corresponding author

Correspondence to Gennady Evtugyn.

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Ivanov, A., Evtugyn, G., Budnikov, H. et al. Cholinesterase sensors based on screen-printed electrodes for detection of organophosphorus and carbamic pesticides. Anal Bioanal Chem 377, 624–631 (2003). https://doi.org/10.1007/s00216-003-2174-9

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  • DOI: https://doi.org/10.1007/s00216-003-2174-9

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