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Quantitative determination of saturated oligogalacturonic acids in enzymatic digests of polygalacturonic acid, pectin and carrot pomace by on-line LC-ESI-MS

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Abstract

A method for the quantitative determination of oligogalacturonic acids (OGAs) by on-line high-performance liquid chromatographic (HPLC) separation and mass spectrometric detection via an electrospray interface (ESI-MS) without additional desalting steps was developed. Saturated OGAs up to a degree of polymerization (dp) of 3 were quantified by comparison with reference compounds. The calibration plots showed high linearity (R 2>0.99), and the detection limits for dp 1, 2, and 3 were 11, 28, and 6 ng per injection, respectively. Non- and partially methyl-esterified OGAs with a dp of 3 and 4 were calculated semi-quantitatively as dp 3. The analytical system was used for the quantification of OGAs of digests obtained by incubation of polygalacturonic acid, pectin, and carrot pomace with commercial enzyme preparations. Furthermore, methyl-esterified OGAs up to a dp of 12 containing up to 4 methyl esters were detected in a pectin digest.

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Acknowledgements

The present work was supported by the Federal Department of Education and Research (BMBF 0339820). The authors would like to thank Dr K.M. Biemel (Institute of Food Chemistry, Hohenheim University) for performing mass spectrometric analyses and Mrs S. Bayha for technical assistance.

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  1. Institute of Food Technology, Section Plant Foodstuff Technology, Hohenheim University, Garbenstrasse 25, 70599, Stuttgart, Germany

    Thomas Stoll, Andreas Schieber & Reinhold Carle

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  1. Thomas Stoll
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  2. Andreas Schieber
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  3. Reinhold Carle
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Correspondence to Andreas Schieber.

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Stoll, T., Schieber, A. & Carle, R. Quantitative determination of saturated oligogalacturonic acids in enzymatic digests of polygalacturonic acid, pectin and carrot pomace by on-line LC-ESI-MS. Anal Bioanal Chem 377, 655–659 (2003). https://doi.org/10.1007/s00216-003-2138-0

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  • DOI: https://doi.org/10.1007/s00216-003-2138-0

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