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An integrated electrochemical fructose biosensor based on tetrathiafulvalene-modified self-assembled monolayers on gold electrodes

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Abstract

An integrated amperometric fructose biosensor based on a gold electrode (AuE) modified with a self-assembled monolayer (SAM) of 3-mercaptopropionic acid (MPA) on which fructose dehydrogenase (FDH) and the mediator tetrathiafulvalene (TTF) are co-immobilized by cross-linking with glutaraldehyde is reported. Variables concerning the behavior of the biosensor were optimized by taking the slope value obtained for the fructose calibration plot in the 0.1–1.0 mM concentration range as a criterion of selection.

At an applied potential of +0.20 V, a good repeatability of such slope values (RSD=6.7%, n=10) was obtained with no need to apply a cleaning or pretreatment procedure to the modified electrode. Moreover, results from five different TTF-FDH-MPA-AuEs yielded a RSD of 5.8%. The useful lifetime of one single biosensor was approximately 30 days, exhibiting a 93% of the original response on the 33rd day. A linear calibration graph was obtained for fructose over the 1.0×10−5–1.0×10−3 M range, with a limit of detection of 2.4×10−6 M. The effect of potential interferents was evaluated. The TTF-FDH-MPA-AuE also performed well in the flow-injection mode. The biosensor was used for the determination of fructose in real samples, and the results compared with those provided by using a commercial enzyme test kit.

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Acknowledgements

The financial support of the Ministerio de Ciencia y Tecnología, Dirección General de Investigación, Project BIO2000–0928 is gratefully acknowledged. S. Campuzano acknowledges a pre-PhD fellowship of the Universidad Complutense de Madrid.

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Authors and Affiliations

  1. Dpto. Química Analítica, Facultad de CC. Químicas, Universidad Complutense de Madrid, 28040, Madrid, Spain

    Susana Campuzano, Rocío Gálvez, María Pedrero, F. Javier Manuel de Villena & José M. Pingarrón

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  1. Susana Campuzano
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  2. Rocío Gálvez
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  3. María Pedrero
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  4. F. Javier Manuel de Villena
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  5. José M. Pingarrón
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Correspondence to José M. Pingarrón.

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Campuzano, S., Gálvez, R., Pedrero, M. et al. An integrated electrochemical fructose biosensor based on tetrathiafulvalene-modified self-assembled monolayers on gold electrodes. Anal Bioanal Chem 377, 600–607 (2003). https://doi.org/10.1007/s00216-003-2123-7

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  • DOI: https://doi.org/10.1007/s00216-003-2123-7

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