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Comparison of biosensors based on entrapment of cholesterol oxidase and cholesterol esterase in electropolymerized films of polypyrrole and diaminonaphthalene derivatives for amperometric determination of cholesterol

Analytical and Bioanalytical Chemistry volume 377, pages 273–280 (2003)Cite this article

Abstract

Cholesterol amperometric biosensors constructed with enzymes entrapped in electropolymerized layers of polypyrrole and poly-naphthalene derivative polymers are compared. The biosensors are based on entrapment of cholesterol oxidase and/or cholesterol esterase in monolayer or multilayer films electrochemically synthesised from pyrrole, 1,8-diaminonaphthalene (1,8-DAN), and 1,5-diaminonaphthalene (1,5-DAN) monomers. Seven configurations were assayed and compared, and different analytical properties were obtained depending on the kind of polymer and the arrangement of the layers. The selectivity properties were evaluated for the different monolayer and bilayer configurations proposed as a function of the film permeation factor. All the steps involved in the preparation of the biosensors and determination of cholesterol were carried out in a flow system. Sensitivity and selectivity depend greatly on hydrophobicity, permeability, compactness, thickness, and the kind of the polymer used. In some cases a protective outer layer of non-conducting poly(o-phenylenediamine) polymer improves the analytical characteristics of the biosensor. A comparative study was made of the analytical performance of each of the configurations developed. The biosensors were also applied to the flow-injection determination of cholesterol in a synthetic serum.

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Acknowledgements

This work was financially supported by the DGI of the Spanish Ministry of Science and Technology, in the framework of the Project BQU2002/02240.

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  1. Analytical Chemistry Department, Faculty of Science, University of Zaragoza, Plaza San Francisco s/n, 50009, Zaragoza, Spain

    J. C. Vidal, E. Garcia-Ruiz, J. Espuelas, T. Aramendia & J. R. Castillo

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  1. J. C. Vidal
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  2. E. Garcia-Ruiz
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  3. J. Espuelas
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  4. T. Aramendia
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  5. J. R. Castillo
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Correspondence to J. C. Vidal.

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Vidal, J.C., Garcia-Ruiz, E., Espuelas, J. et al. Comparison of biosensors based on entrapment of cholesterol oxidase and cholesterol esterase in electropolymerized films of polypyrrole and diaminonaphthalene derivatives for amperometric determination of cholesterol. Anal Bioanal Chem 377, 273–280 (2003). https://doi.org/10.1007/s00216-003-2120-x

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  • DOI: https://doi.org/10.1007/s00216-003-2120-x

Keywords

  • Multilayer amperometric biosensor
  • Enzyme entrapment
  • Electropolymerization
  • Polypyrrole
  • Substituted naphthalene monomers
  • Cholesterol determination