New methods for acceleration of meat sample preparation prior to determination of the metal content by atomic absorption spectrometry

Abstract

Focused microwave-assisted digestion and ultrasound leaching have been applied for the extraction of Pb, Cd, Cr, Cu, Fe, Zn, Ca, and Mg from raw meat. Semimembranous muscle (SM) of raw pig ham was used for optimizing both the digestion and extraction steps by multivariate approaches. The detection and quantification limits were 0.5 and 0.9 μg kg⁻¹ for Pb, 0.06 and 0.1 μg kg⁻¹ for Cd, 0.2 and 1.2 μg kg⁻¹ for Cr, 0.4 and 3 μg kg⁻¹ for Cu, 0.04 and 0.1 mg kg⁻¹ for Fe, 0.012 and 0.017 mg kg⁻¹ for Zn, 0.3 and 0.4 mg kg⁻¹ for Ca, and 0.01 and 0.03 mg kg⁻¹ for Mg. The precision, expressed as relative standard deviation (RSD), ranged between 2.5 and 9.6% for focused microwave-assisted digestion and between 3.5 and 10.6% for ultrasound leaching. The methods were then compared with a reference method and applied to a certified reference material (bovine muscle 184, from the BCR). The t-test, applied to the results obtained from focused microwave-assisted digestion, revealed that they are in agreement (p>0.01) with the certified and estimated values in the case of Pb, Cd, Cr, Cu, Fe, Ca, Mg, and Zn but not in that of Fe. In the case of ultrasound leaching, only the extraction of Pb, Cu, and Ca was quantitative. The method based on microwave digestion provides more accurate and precise results than ultrasound leaching. These new procedures have many advantages with regards to conventional methods, namely, reduction of the extraction time, simplification of the process, avoidance of chemical emissions to the atmosphere, and no losses of metals by volatilization.