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Precision studies using the ABI Prism 3100 Genetic Analyzer for forensic DNA analysis

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Abstract

Precision studies using the ABI Prism 3100 Genetic Analyzer have been conducted by performing multiple runs of the AmpFℓSTR Identifiler allelic ladder, 9947A kit positive control DNA and the 250-base-pair fragment from the internal size standard (GeneScan LIZ-500). Intra-run and inter-run precision data demonstrates the sizing reproducibility of the 3100 instrument for forensic applications. This precision data is utilized to assist in making allele assignments for outlier and variant alleles (i.e., 'off-ladder' alleles). It also provides a means of addressing systematic variations due to temperature fluctuations, polymer (POP-4) or capillary effects, and associated troubleshooting efforts.

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Acknowledgements

The authors would like to thank Mr Brian Heaton for his invaluable graphing assistance.

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Authors and Affiliations

  1. Massachusetts State Police Crime Laboratory, Sudbury, MA, USA

    Joanne B. Sgueglia, Stephana Geiger & Jeffrey Davis

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  1. Joanne B. Sgueglia
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  2. Stephana Geiger
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  3. Jeffrey Davis
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Correspondence to Joanne B. Sgueglia.

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Sgueglia, J.B., Geiger, S. & Davis, J. Precision studies using the ABI Prism 3100 Genetic Analyzer for forensic DNA analysis. Anal Bioanal Chem 376, 1247–1254 (2003). https://doi.org/10.1007/s00216-003-1998-7

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  • DOI: https://doi.org/10.1007/s00216-003-1998-7

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