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Determination of copper, nickel, cobalt, silver, lead, cadmium, and mercury ions in water by solid-phase extraction and the RP-HPLC with UV-Vis detection

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Abstract

A new method for the simultaneous determination of seven heavy metal ions in water by solid-phase extraction and reversed-phase high-performance liquid chromatography (RP-HPLC) was developed. The copper, nickel, cobalt, silver, lead, cadmium, and mercury ions were pre-column derivatized with tetra(m-aminophenyl)porphyrin (Tm-APP) to form colored chelates. The metal–Tm-APP chelates in 100 mL of sample were preconcentrated to 1 mL by solid-phase extraction with a C18 cartridge; an enrichment factor of 100 was achieved. The chelates were separated on a Waters XterraRP18 column by gradient elution with methanol (containing 0.05 mol L−1 pyrrolidine–acetic acid buffer salt, pH 10.0) and acetone (containing 0.05 mol L−1 pyrrolidine–acetic acid buffer salt, pH 10.0) as mobile phase at a flow rate of 1.0 mL min−1 and detected with a photodiode array detector. The detection limits of copper, cobalt, nickel, silver, lead, cadmium, and mercury are 2, 2, 3, 4, 3, 3, and 3 ng L−1, respectively, in the original sample. The method was also applied to the determination of these metals in water with good results.

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Authors and Affiliations

  1. Department of Chemistry, Yunnan University, 650091, Kunming, P.R. China

    Qiufen Hu, Guangyu Yang, Yiyun Zhao & Jiayuan Yin

  2. Department of Chemistry, Yuxi Teacher's College, 653100, Yuxi, P.R. China

    Qiufen Hu

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  1. Qiufen Hu
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  2. Guangyu Yang
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  3. Yiyun Zhao
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  4. Jiayuan Yin
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Correspondence to Guangyu Yang.

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Hu, Q., Yang, G., Zhao, Y. et al. Determination of copper, nickel, cobalt, silver, lead, cadmium, and mercury ions in water by solid-phase extraction and the RP-HPLC with UV-Vis detection. Anal Bioanal Chem 375, 831–835 (2003). https://doi.org/10.1007/s00216-003-1828-y

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  • DOI: https://doi.org/10.1007/s00216-003-1828-y

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