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Enantioselective separations in capillary electrophoresis with dextran sulfate as the chiral selector

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Abstract

Dextran sulfate, a polyanionic polysaccharide, was evaluated as a chiral additive in capillary electrophoresis. Structurally related compounds having a variety of functional groups were utilized to probe the selectivity of the chiral selector. The effects of pH, chiral selector concentration, and chiral selector composition on resolution were also studied. At low pH, the reversed polarity mode was employed to achieve separation of the probe compounds. The electrophoretic results provided insight into the chiral recognition of dextran sulfate in capillary electrophoresis. Several factors, including hydrophobic, steric, and electrostatic interactions, appeared to play a role in the observed enantioseparations.

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Acknowledgement

The authors gratefully acknowledge financial support for this work from the Advanced Technology Program at the National Institute of Standards and Technology.

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Authors and Affiliations

  1. Analytical Chemistry Division, Chemical Science and Technology Laboratory, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA

    Karen W. Phinney & Lane C. Sander

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  1. Karen W. Phinney
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  2. Lane C. Sander
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Correspondence to Karen W. Phinney.

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Contribution of the National Institute of Standards and Technology. Not subject to copyright.

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Phinney, K.W., Sander, L.C. Enantioselective separations in capillary electrophoresis with dextran sulfate as the chiral selector. Anal Bioanal Chem 375, 763–768 (2003). https://doi.org/10.1007/s00216-003-1808-2

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  • DOI: https://doi.org/10.1007/s00216-003-1808-2

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