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Preparation of weak cation exchange packings based on monodisperse poly(glycidyl methacrylate-co-ethylene dimethacrylate) beads and their chromatographic properties

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Abstract

The monodisperse, macroporous poly(glycidyl methacrylate-co-ethylene dimethacrylate) beads were synthesized by a single-step swelling and polymerization method. Based on this media, a weak cation exchange (WCX) stationary phase for HPLC was synthesized by a new chemically modified method. The prepared resin has advantages for biopolymer separation, high column efficiency, low column backpressure, high protein mass recovery, and good resolution for proteins. The measured bioactivity recovery for lysozyme was 98±5%. The dynamic protein loading capacity of the WCX packings was 17.3 mg g−1. The experimental results show that the synthesized WCX resin has very weak hydrophobicity.

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Acknowledgments

Financial support of this research by the National Scientific Foundation in China (Nos. 39880003 and 20175016) are gratefully acknowledged.

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Authors and Affiliations

  1. Institute of Modern Separation Science, Key Laboratory of Modern Separation Science in Shannxi Province, Northwest University, 710069, Xi'an, P.R. China

    Bolin Gong, Congyu Ke & Xindu Geng

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  1. Bolin Gong
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  2. Congyu Ke
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  3. Xindu Geng
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Correspondence to Xindu Geng.

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Gong, B., Ke, C. & Geng, X. Preparation of weak cation exchange packings based on monodisperse poly(glycidyl methacrylate-co-ethylene dimethacrylate) beads and their chromatographic properties. Anal Bioanal Chem 375, 769–774 (2003). https://doi.org/10.1007/s00216-003-1805-5

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  • DOI: https://doi.org/10.1007/s00216-003-1805-5

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