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Gramicidin A interaction at a dioleoyl phosphatidylcholine monolayer on a mercury drop electrode

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Abstract

A biosensor where the sensing surface is a fluid dioleyl phosphatidylcholine monolayer (DOPC) deposited on a mercury drop was used. The lipid monolayer was held in 0.1 M NaCl and a concentration of gramicidin A in the range 0–12 nM was used. Electrochemical impedance spectroscopy in the frequency range 0.1–65 kHz was employed to investigate how the defect-free monolayer responds to interactions of gramicidin A in solution.

The data was analyzed both with multivariate data analysis and classical electrochemical methods. The principal component analysis of the resulting impedance spectra gave a linear dependence on the concentration of gramicidin A. An increasing permittivity was observed in the low-frequency regime with increasing concentration of gramicidin A in solution.

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Acknowledgement

The authors in Umeå thank European Union Structure Foundation Objective 1 for financial support and Andrew Nelson the MoD Dstl (UK) for funding.

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Authors and Affiliations

  1. Department of Chemistry, Umeå University, 90187, Umeå, Sweden

    Britta Lindholm-Sethson & Josefina Nyström

  2. The Unit of Biomass Technology and Chemistry, SLU Röbäcksdalen, P.O. Box 4097, 90403, Umeå, Sweden

    Paul Geladi

  3. Self-Organising Molecular Systems, Department of Chemistry, University of Leeds, LS2 9JT, UK

    Andrew Nelson

Authors
  1. Britta Lindholm-Sethson
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  2. Josefina Nyström
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  3. Paul Geladi
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  4. Andrew Nelson
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Correspondence to Britta Lindholm-Sethson.

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Lindholm-Sethson, B., Nyström, J., Geladi, P. et al. Gramicidin A interaction at a dioleoyl phosphatidylcholine monolayer on a mercury drop electrode. Anal Bioanal Chem 375, 350–355 (2003). https://doi.org/10.1007/s00216-002-1697-9

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  • DOI: https://doi.org/10.1007/s00216-002-1697-9

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