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Laser-induced fluorescence with an OPO system. Part I. Optimisation of the analytical system by use of experimental design methodology. Application to the direct quantification of traces of benzo[a]pyrene

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This study deals with the optimisation and application of a method for direct analysis of trace pollutants in water by laser-induced fluorescence. The arrangement used consisted of an Nd:YAG Laser coupled with an optical parametric oscillator (LYOPO) and connected to a spectrophotometer and a high-sensitivity camera. Optimisation was achieved by developing an experimental design methodology to maximise the signal-to-noise ratio and reduce the limit of detection. The technique was then applied to the detection of benzo[a]pyrene in water. The experimental results were evidence of its high sensitivity and time-resolution potential. The detection limit for benzo[a]pyrene was 0.7 ng L–1 in drinking water and 4 ng L–1 in raw water containing 1 mg L–1 humic acids.

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Giamarchi, .P., Burel, .L., Stephan, .L. et al. Laser-induced fluorescence with an OPO system. Part I. Optimisation of the analytical system by use of experimental design methodology. Application to the direct quantification of traces of benzo[a]pyrene . Anal Bioanal Chem 374, 490–497 (2002). https://doi.org/10.1007/s00216-002-1472-y

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  • DOI: https://doi.org/10.1007/s00216-002-1472-y

  • Laser-induced fluorescence (LIF) Time-resolved fluorescence Direct determination Optimisation Experimental design Polycyclic aromatic hydrocarbons (PAH) Ultra-trace quantification