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Amperometric determination of glutathione and cysteine on a Pd-IrO2 modified electrode with high performance liquid chromatography in rat brain microdialysate

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Abstract.

A Pd/IrO2 co-electrodeposited glassy carbon electrode was prepared and the electrochemical behavior of glutathione (GSH) at this chemically modified electrode (CME) has been studied by cyclic voltammetry (CV). The results indicated that the modified electrode efficiently exhibited electrocatalytic oxidation for GSH with relatively high sensitivity, stability, and long-life. Coupled with high-performance liquid chromatography (HPLC), the Pd/IrO2 modified electrode was utilized for the electrochemical detection (ECD) of the thiocompounds, glutathione and cysteine (Cys). The peak currents were linear with the substance concentrations in the range of 1.0×10–5 mol L–1 to 8.0×10–4 mol L–1 for GSH and 4.0×10–6 mol L–1 to 2.0×10–4 mol L–1 for Cys. The detection limits were 2.0×10–6 mol L–1 for GSH and 5.0×10–7 mol L–1 for Cys with S/N of 3. The method has been successfully applied to assess the contents of GSH and Cys in rat brain microdialysates.

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Authors and Affiliations

  1. Department of Chemistry, East China Normal University, Shanghai, 200062, P. R. China, P.R. China

    Fang Xu, Lin Wang, Mengnan Gao & Litong Jin

  2. Department of Chemistry, Faculty of Engineering, Gifu University, Gifu 501–11, Japan, Japan

    Jiye Jin

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  1. Fang Xu
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  2. Lin Wang
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  3. Mengnan Gao
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  4. Litong Jin
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  5. Jiye Jin
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Xu, F., Wang, L., Gao, M. et al. Amperometric determination of glutathione and cysteine on a Pd-IrO2 modified electrode with high performance liquid chromatography in rat brain microdialysate. Anal Bioanal Chem 372, 791–794 (2002). https://doi.org/10.1007/s00216-002-1255-5

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  • DOI: https://doi.org/10.1007/s00216-002-1255-5

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