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Region-dependent regulation of acute ethanol on γ oscillation in the rat hippocampal slices

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Abstract

Background

Ethanol use disorders are a serious medical and public health problem in the world today. Acute ethanol intoxication can lead to cognitive dysfunction such as learning and memory impairment. Gamma oscillations (γ, 30–80 Hz) are synchronized rhythmic activity generated by population of neurons within local network, and closely related to learning and memory function. The hippocampus is a critical anatomic structure that supports learning and memory. On the grounds of structure and function, hippocampus can be divided into the intermediate (IH), the dorsal (DH), and ventral hippocampus (VH). The current study is the first to investigate the effects of acute ethanol on γ oscillations in these sub-regions of rat hippocampal slices.

Methods

The sustained γ oscillations were induced by 200 nM kainate (KA) in the CA3c of IH, DH, and VH. When KA-induced γ oscillation reached the steady state, ethanol (50 mM or 100 mM) was applied and the effects of ethanol on γ oscillation power was measured in the slices sequentially sectioned from ventral to dorsal hippocampus of adult rats.

Results

In the intermediate hippocampal slices, compared with control (KA only), ethanol (50 mM) caused 36.1 ± 3.9% decrease in γ power (p < 0.05, n = 10), while ethanol (100 mM) caused 55.3 ± 5.5% decrease in γ power (p < 0.001, n = 14). In the dorsal hippocampus, only ethanol (100 mM) caused 18.1 ± 8.6% decrease in γ power (p < 0.05, n = 12). However, in the ventral hippocampus, neither 50 mM nor 100 mM ethanol affected γ oscillation.

Conclusions

Our results demonstrate that ethanol may produce the differential suppression of γ oscillations in a dose-dependent manner in different sub-regions of hippocampus, suggesting that the modulation of ethanol on hippocampal γ oscillation is region-dependent.

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Funding

This study was supported by the National Natural Science Foundation of China (NSFC, grant numbers: 81271422; U1804170; 81100953; 1070938), International Collaboration of Henan Province Science-Technique Bureau (134300510040), Natural Science Foundation of Hebei Province (H2012203067) and Key Program for Applied Basic Research of Hebei Key Program for Applied Basic Research of Hebei Province (12966119D).

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Authors and Affiliations

  1. The Second Affiliated Hospital of Xinxiang Medical University, Xinxiang Medical University, Xinxiang, 453003, China

    Zhihua Liu, Bin Yang, Xiangyang Zhang, Chengbiao Lu & Ruiling Zhang

  2. Key Laboratory for the Brain Research of Henan Province/The International-Joint Lab for Non-invasive Neural Modulation, School of Basic Medical Sciences, Xinxiang Medical University, Xinxiang, 453003, China

    Zhihua Liu, Fangli Guo & Chengbiao Lu

  3. School of Basic Medical Sciences, Xinxiang Medical University, Xinxiang, 453003, China

    Feng Ren

  4. Department of Psychology, Xinxiang Medical University, Xinxiang, 453003, Henan, People’s Republic of China

    Hongxing Zhang

Authors
  1. Zhihua Liu
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  2. Feng Ren
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  3. Bin Yang
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  4. Hongxing Zhang
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  5. Fangli Guo
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  7. Chengbiao Lu
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  8. Ruiling Zhang
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Contributions

ZRL and LCB designed and supervised the experiments; LZH, YB, and GFL performed the experiments; RF, ZHX, ZXY, LZH, and LCB analyzed the data. LCB, LZH, and ZRL wrote and revised the manuscript; All authors reviewed the manuscript.

Corresponding authors

Correspondence to Chengbiao Lu or Ruiling Zhang.

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Liu, Z., Ren, F., Yang, B. et al. Region-dependent regulation of acute ethanol on γ oscillation in the rat hippocampal slices. Psychopharmacology 237, 2959–2966 (2020). https://doi.org/10.1007/s00213-020-05584-y

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  • DOI: https://doi.org/10.1007/s00213-020-05584-y

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